Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

p53 antibody (Tumor Protein P53) (full length) Primary Antibody

TP53 Reactivity: Human, Mouse, Rat IHC (f), IHC (fro), ICS, IP, WB Host: Mouse Monoclonal G59-12 unconjugated
Pubmed (8) Independent Validation (1)
Catalog No. ABIN967416
Contact our Customer Service for availability and price in your country.
  • Target
    p53 (TP53)
    Binding Specificity
    full length
    • 56
    • 45
    • 38
    • 35
    • 34
    • 34
    • 34
    • 32
    • 26
    • 26
    • 25
    • 24
    • 21
    • 19
    • 18
    • 18
    • 16
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 13
    • 11
    • 11
    • 10
    • 10
    • 9
    • 9
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Human, Mouse, Rat
    • 1026
    • 297
    • 263
    • 179
    • 77
    • 64
    • 64
    • 31
    • 14
    • 13
    • 12
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Host
    Mouse
    • 739
    • 317
    • 4
    • 2
    • 1
    • 1
    • 1
    Clonality
    Monoclonal
    • 680
    • 383
    • 1
    Conjugate
    This p53 antibody is un-conjugated
    • 553
    • 63
    • 41
    • 36
    • 30
    • 29
    • 24
    • 23
    • 23
    • 23
    • 23
    • 20
    • 20
    • 13
    • 13
    • 13
    • 13
    • 13
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
    • 738
    • 347
    • 292
    • 275
    • 221
    • 158
    • 157
    • 154
    • 61
    • 52
    • 47
    • 29
    • 28
    • 20
    • 18
    • 17
    • 15
    • 13
    • 12
    • 9
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Brand
    BD Pharmingen™
    Cross-Reactivity
    Mouse (Murine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Recombinant full-length human p53
    Clone
    G59-12
    Isotype
    IgG1
  • Application Notes
    Clone G59-12 conjugated to R-Phycoerythrin (PE) is suggested for flow cytometric analysis of p53. Positive control cell lines include SKBR-3 human breast carcinoma cells (ATCC HTB-30) and A431 human vulval carcinoma cells (ATCC CRL-1555). Jurkat T cells (ATCC TIB-152) or MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as negative controls. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
    Restrictions
    For Research Use only
  • Validation #028755 (Western Blotting)
    'Independent Validation' Badge
    by
    Alamo Laboratories Inc
    No.
    #028755
    Date
    09/16/2013
    Antigen
    Lot Number
    2335876
    Method validated
    Western Blotting
    Positive Control
    U251-MG cells
    Negative Control
    PC3 cells
    Notes
    Strong bands of the expected size were observed in the positive control sample, and not in the negative control sample.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Tumor Protein P53 (TP53) antibody
    • Catalog number: ABIN967416
    • Lot number: 2335876
    Secondary Antibody
    • Antibody: Goat anti-Mouse IgG-HRP
    • Lot number: 0312
    Full Protocol
    • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25%
    • Beta-mercaptoethanol at 95°C for 5 min prior to loading.
    • 2. 32 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
    • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a
    • standard.
    • 4. Proteins were then transferred onto PVDF membrane by tank transfer and protein transfer was
    • confirmed with Ponceau S staining.
    • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room
    • temperature for 1 h.
    • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
    • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1%
    • (W/V) non-fat dry milk and incubated for 2 h at room temperature (~26°C).
    • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing
    • 1% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed thrice PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 11. The membrane was washed in PBS twice for 30 sec each.
    • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The
    • blot was scanned for 300 sec.
    • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.4. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10
    • min each.
    • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
    • 16. Repeated Steps 5-12 with the loading control antibody (beta-actin) and its matching secondary
    • antibody.
    Experimental Notes
    None
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Van Meir, Roemer, Diserens, Kikuchi, Rempel, Haas, Huang, Friedmann, de Tribolet, Cavenee: "Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 4, pp. 1008-12, 1995 (PubMed).

    Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994 (PubMed).

    Mørkve, Halvorsen, Stangeland, Gulsvik, Laerum: "Quantitation of biological tumor markers (p53, c-myc, Ki-67 and DNA ploidy) by multiparameter flow cytometry in non-small-cell lung cancer." in: International journal of cancer. Journal international du cancer, Vol. 52, Issue 6, pp. 851-5, 1993 (PubMed).

    van den Berg, Baas, Polak, Offerhaus: "Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid." in: The American journal of pathology, Vol. 142, Issue 2, pp. 381-5, 1993 (PubMed).

    Yeargin, Cheng, Yu, Gjerset, Bogart, Haas: "P53 mutation in acute T cell lymphoblastic leukemia is of somatic origin and is stable during establishment of T cell acute lymphoblastic leukemia cell lines." in: The Journal of clinical investigation, Vol. 91, Issue 5, pp. 2111-7, 1993 (PubMed).

    Cheng, Yee, Yeargin, Friedmann, Haas: "Suppression of acute lymphoblastic leukemia by the human wild-type p53 gene." in: Cancer research, Vol. 52, Issue 1, pp. 222-6, 1992 (PubMed).

    Yeargin, Cheng, Haas: "Role of the p53 tumor suppressor gene in the pathogenesis and in the suppression of acute lymphoblastic T-cell leukemia." in: Leukemia, Vol. 6 Suppl 3, pp. 85S-91S, 1992 (PubMed).

    Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991 (PubMed).

  • Target
    p53 (TP53)
    Alternative Name
    p53 (TP53 Antibody Abstract)
    Synonyms
    BCC7, LFS1, P53, TRP53, p53, Trp53, brp53, drp53, etID22686.5, fb40d06, wu:fb40d06, zgc:111919, Tp53, bbl, bfy, bhy, p44, tp53, Xp53, TP53, tumor protein p53, cellular tumor antigen p53, transformation related protein 53, tumor protein p53 L homeolog, TP53, CpipJ_CPIJ002758, Tp53, tp53, Trp53, tp53.L
    Background
    P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and the wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the S-phase of the cell cycle. p53 migrates at a reduced molecular weight of 53 kDa. Clone G59-12 recognizes mutant and wild type human, rat and mouse p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen.
    Molecular Weight
    53 kDa
    Pathways
    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
You are here:
help Support