GFAP antibody
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- Target See all GFAP Antibodies
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Reactivity
- Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit, Guinea Pig, Sheep, Dog
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GFAP antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- BD Pharmingen™
- Cross-Reactivity
- Rat (Rattus), Mouse (Murine), Human, Sheep (Ovine), Dog (Canine), Pig (Porcine), Rabbit, Guinea Pig, Chicken
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Bovine spinal cord homogenate
- Clone
- 2E1
- Isotype
- IgG2b
- Top Product
- Discover our top product GFAP Primary Antibody
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- Application Notes
- Pharmingen offers additional GFAP specific antibodies: clone 1B4 (ABIN967500), 4A11 (ABIN967499), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502). Applications include immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (5-25 µg/ml), and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control.
- Comment
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Related Products: ABIN967502, ABIN967499
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4° C.
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Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).
: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).
: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).
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Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).
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- Target
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Alternative Name
- GFAP (GFAP Products)
- Synonyms
- GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
- Background
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GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen for clone 2E1. This antibody has broad species reactivity. It recognizes GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 2E1 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells.
The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure). - Molecular Weight
- 50 kDa
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