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CDK4 antibody (AA 1-303)

CDK4 Reactivity: Human, Mouse, Rat WB, IP, IF, IHC (f) Host: Mouse Monoclonal 97-Cdk4 unconjugated
Catalog No. ABIN967778
  • Target See all CDK4 Antibodies
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Binding Specificity
    • 23
    • 16
    • 15
    • 9
    • 9
    • 9
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 1-303
    Reactivity
    • 117
    • 73
    • 53
    • 19
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 108
    • 31
    • 2
    • 1
    Mouse
    Clonality
    • 107
    • 34
    Monoclonal
    Conjugate
    • 81
    • 11
    • 11
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CDK4 antibody is un-conjugated
    Application
    • 119
    • 55
    • 37
    • 31
    • 18
    • 18
    • 16
    • 15
    • 14
    • 13
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Cross-Reactivity
    Mouse (Murine), Human
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat Cdk4 aa. 1-303
    Clone
    97-Cdk4
    Isotype
    IgG1
    Top Product
    Discover our top product CDK4 Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Angus, Wheeler, Ranmal, Zhang, Markey, Mathews, Knudsen: "Retinoblastoma tumor suppressor targets dNTP metabolism to regulate DNA replication." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44376-84, (2002) (PubMed).

    Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

    Bagui, Jackson, Agrawal, Pledger: "Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1)." in: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8748-57, (2000) (PubMed).

    Kato, Matsushime, Hiebert, Ewen, Sherr: "Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4." in: Genes & development, Vol. 7, Issue 3, pp. 331-42, (1993) (PubMed).

  • Target
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Alternative Name
    Cdk4 (CDK4 Products)
    Synonyms
    zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody
    Background
    The Cdk4 kinase is a cell cycle-regulated cyclin-dependent kinase that associates with cyclins D1, D2, and D3. In macrophages and fibroblasts, Cdk4-Cyclin D1 complexes are predominant. Much like Cdk2 and Cdc2, the activity of Cdk4 is regulated by association with its respective kinase and by phosphorylation of specific threonine residues. The cdk-activating kinase (CAK) is a regulator of cdk-cyclin activity by virtue of its ability to phosphorylate single threonine residues on Cdk2, Cdc2, and Cdk4. Active Cdk4-Cyclin D1 complexes are capable of phosphorylating the retinoblastoma gene product (pRb), but not histone H1 or casein. Formation of the Cdk4-Cyclin D holoenzyme and phosphorylation of the catalytic subunit appear to be independently regulated. Therefore, other cellular factors may be necessary for stabilization of this protein complex in order to facilitate the phosphorylation of Cdk4. An inhibitor of Cdk4 kinase activity, known as p16, has been identified and partially characterized. The p16 gene appears to be altered in many tumors, suggesting that this is a tumor suppressor gene. It is thought that p16 inhibition of Cdk4-Cyclin D interferes with cell cycle progression by preventing phosphorylation of pRb by Cdk4 and the subsequent release of factors that activate transcription. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    33 kDa
    Pathways
    Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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