BAX antibody (AA 55-178)

Catalog No. ABIN968294

Product Details anti-BAX Antibody

Target: BAX (BCL2-Associated X Protein (BAX))

Binding Specificity: AA 55-178

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Bax-alpha

Clone: 3

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968587, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 µg/ml

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-BAX antibody (ABIN968294)

Kartasheva, Contente, Lenz-Stöppler, Roth, Dobbelstein: "p53 induces the expression of its antagonist p73 Delta N, establishing an autoregulatory feedback loop." in: Oncogene, Vol. 21, Issue 31, pp. 4715-27, (2002) (PubMed).

Aoudjit, Vuori: "Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cells." in: Oncogene, Vol. 20, Issue 36, pp. 4995-5004, (2001) (PubMed).

Rytömaa, Lehmann, Downward: "Matrix detachment induces caspase-dependent cytochrome c release from mitochondria: inhibition by PKB/Akt but not Raf signalling." in: Oncogene, Vol. 19, Issue 39, pp. 4461-8, (2000) (PubMed).

Xu, Reed: "Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast." in: Molecular cell, Vol. 1, Issue 3, pp. 337-46, (1998) (PubMed).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax (BAX Products)

Synonyms: BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody

Background: Proper development and maintenance of homeostasis within tissues is achieved through cell proliferation, differentiation, and apoptosis. Members of the Bcl-2 protein family function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Similar to other family members, Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains which allow for its homodimerization or heterodimerization with Bcl-2. Although Bax alone does not induce cell death, elevated levels of Bax accelerate apoptosis following a death signal such as cytokine deprivation or Fas/Fas ligand interaction. The ratio of Bax homodimers to Bax/Bcl-2 heterodimers is an apoptotic checkpoint. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death. Bax function is also affected by Bax Inhibitor-1 (BI-1). Although it interacts directly with Bcl-2 and not Bax, BI-1 can suppress Bax-induced apoptosis. Alternative splicing of Bax mRNA produces Bax-alpha, the integral membrane form, and the two cytosolic forms beta and gamma. These isoforms are thought to allow for differential regulation of Bax activity and localization. This antibody is routinely tested by western blot analysis.

Molecular Weight: 21 kDa

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response