JNK2 antibody (AA 217-230)

Catalog No. ABIN987273

The Goat Polyclonal anti-JNK2 antibody (ABIN987273) specifically detects JNK2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for JNK2 antibody (AA 217-230) (ABIN987273)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This JNK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-JNK2 Antibody

Binding Specificity: AA 217-230

Purpose: MAPK9 / JNK2 alpha (aa217-230)

Sequence: ELVKGCVIFQ GTDH

Specificity: This antibody is expected to recognize isoforms alpha1, alpha 2. and gamma (NP_620707.1, NP_002743.3, NP_001128516.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ELVKGCVIFQGTDH, from the internal region of the protein sequence according to NP_620707.1, NP_002743.3, NP_001128516.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell line HeLa (calculated MW of 48.1 kDa according to NP_002743.3). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Alternative Name: MAPK9

Background: MAPK9, mitogen-activated protein kinase 9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK, Jun kinase, MAP kinase 9, MAPK 9, OTTHUMP00000161542, OTTHUMP00000161543, c-Jun N-terminal kinase 2, c-Jun kinase 2, stress-activated

Gene ID: 5601, 26420, 50658

NCBI Accession: NP_620707, NP_002743, NP_001128516

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins