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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

All Flow Cytometry Antibodies

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83,890 results

ICAM1 antibody (AA 201-300)

ICAM1 Reactivity: Human, Mouse, Rat, Rabbit WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (ABIN670806), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

16 references

Catalog No. ABIN670806

Details

alpha Tubulin antibody (FITC)

TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Turkey, Paramecium ICC, FACS Host: Mouse Monoclonal TU-01 FITC

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/mL, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/mL, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

FACS

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red).

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red).

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

FACS

ICC

8 images

11 references

Catalog No. ABIN93892

Details

beta Actin antibody

ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

All lanes : Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded H.

IHC (p)

Confocal immunofluorescent analysis of ACTB Antibody (ABIN658990 and ABIN2838035) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of anti-ACTB Antibody (ABIN658990 and ABIN2838035) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane).

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

IHC (p)

7 images

12 references

Catalog No. ABIN658990

Details

TUBB3 antibody (N-Term) (FITC)

TUBB3 Reactivity: Human, Mouse, Rat, Dog, Pig FACS, ICC Host: Mouse Monoclonal TU-20 FITC

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml).

Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μL reagent per million cells in 100 μL of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/mL, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.

FACS

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml).

ICC

6 images

10 references

Catalog No. ABIN93912

Details

FcRn antibody

FcRn Reactivity: Human ELISA, IF, FACS, cELISA, Func Host: Mouse Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity.

8 images

16 references

Catalog No. ABIN1774762

Details

Liver Arginase antibody

KO Validated ARG1 Reactivity: Human WB, ELISA, FACS, IHC (p), IF, IP, ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE antibody diluted at 1:50000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I , antibody at 1:200 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I , antibody at 1:500 dilution.

IHC

WB Image Arginase 1 antibody detects Arginase 1 protein by western blot analysis.

Elevated right ventricular pressure, increased right ventricular weight and altered expression of Ddah1 and Arg1 in cavin-/- mice.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Arg-1 expression in neutrophils and lymph-node microenvironmentNeutrophils isolated from control and HL patients were analysed for Arg-1 expression (A), activity by measuring urea (B) and protein expression by Western Blot (C).

8 images

11 references

Catalog No. ABIN2856661

Details

SQSTM1 antibody

KO Validated SQSTM1 Reactivity: Human WB, IF, IP, ICC, FACS, IHC (p), PLA Host: Rabbit Polyclonal unconjugated

IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.

IHC

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at 1:1000

IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal, Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

FACS

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

HDAC2 positively regulates the expression of p-Akt, p-mTOR, and survivin in ER+ breast cancer cells.

Effect of autophagy on miR-21-induced drug resistance.

Celastrol activates autophagy and inhibits MPP+-induced neurotoxicity in dopaminergic neurons.

Celastrol enhances the clearance of damaged mitochondria via activating mitophagy.

23 images

13 references

Catalog No. ABIN2854836

Details

RUNX3 antibody (AA 31-130)

RUNX3 Reactivity: Human WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Runx3 Polyclonal Antibody, Unconjugated (ABIN1714069) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

5 images

1 validation

Catalog No. ABIN1714069

Details

Independently Validated

TTF1 antibody (AA 201-300)

TTF1 Reactivity: Human, Rat, Mouse WB, FACS, ICC, IF (cc) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

SDS

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

IF (cc)

4 images

2 references

1 validation

Catalog No. ABIN728713

Details

Independently Validated

IGF1R antibody (AA 251-350)

IGF1R Reactivity: Human, Mouse, Rat, Dog FACS Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody (ABIN726575)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .

FACS

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

2 references

1 validation

Catalog No. ABIN726575

Details

Independently Validated

CDH6 antibody (AA 281-380)

CDH6 Reactivity: Human WB, ELISA, FACS Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

5 images

1 validation

Catalog No. ABIN715286

Details

Independently Validated

FZD7 antibody (AA 501-574)

FZD7 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 501-574) antibody (ABIN710051)

Image provided by the Independent Validation Program (badge number 29805).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

4 images

1 reference

1 validation

Catalog No. ABIN710051

Details

Independently Validated

MTOR antibody (AA 2436-2492)

mTOR Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody (ABIN676403)

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated and DAPI staining

IHC

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

9 references

1 validation

Catalog No. ABIN676403

Details

Independently Validated

Cadherin antibody (AA 841-882)

Reactivity: Human, Mouse WB, FACS, IF (cc), IF (p), IHC (p), ELISA, IHC (fro), ICC Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

FACS

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

4 images

2 references

1 validation

Catalog No. ABIN687682

Details

Independently Validated

LRP2 antibody (AA 3401-3500) (Cy3)

LRP2 Reactivity: Human, Mouse FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Cy3

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

5 images

1 validation

Catalog No. ABIN750991

Details

Independently Validated

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