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Western Blot Antibodies

Western blot (WB) antibodies are optimized for the detection of denatured proteins and validated to deliver clear, specific bands at the expected molecular weight. Use the filters below to quickly identify primary antibodies matched to your target, species, and experimental requirements.

Western Blot Antibody Validation

Antibodies for Western blot must reliably detect target proteins under denaturing conditions. Products in this category are validated for WB applications and selected for specific band detection at the expected molecular weight. Where available, validation data include experimental images, independent testing, and literature references to support antibody performance.

Western Blot Considerations

Protein denaturation during Western blotting can alter epitope structure, making antibody selection critical. Antibodies validated for WB are optimized to recognize linearized epitopes and reduce non-specific binding.

Monoclonal antibodies provide high specificity and consistent results, while polyclonal antibodies can offer stronger signal due to multi-epitope recognition. Recombinant antibodies combine defined specificity with improved reproducibility across experiments.

Western Blotting: Principles, Protocols, and Troubleshooting

Learn more about Western blotting, including protocols and troubleshooting

Use the filters below to narrow down Western blot antibodies by target, species, and experimental requirements.

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529,062 results

GAPDH antibody (C-Term)

GAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane).

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram).

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b.

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

7 images

1 reference

Catalog No. ABIN392354

$451.00

Plus shipping costs $50.00

400 μL (More quantities available)

Shipping to: United States

Delivery in 10 to 12 Business Days

Details

PCNA antibody (AA 53-196)

PCNA Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7H4F8 unconjugated

Western blot analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA mouse mAb with DAB staining.

IHC

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.

Western blot analysis using PCNA mAb against human PCNA recombinant protein.

Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA mouse mAb with DAB staining.

IHC

7 images

2 references

Catalog No. ABIN1724760

$548.16

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 5 Business Days

Details

PCNA antibody (AA 53-196)

PCNA Reactivity: Human, Monkey WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7H4F8 unconjugated

IHC

FACS

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS7 (7), and MCF-7 (8) cell lysate.

IHC

7 images

2 references

Catalog No. ABIN1724759

$548.16

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 5 Business Days

Details

PCNA antibody (AA 151-261)

PCNA Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse spleen lysates probed with PCNA Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

6 references

Catalog No. ABIN676583

$129.85

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 7 Business Days

Details

PCNA antibody (AA 1-261)

PCNA Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 1G7 unconjugated

PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in Hela .

Immunofluorescence of monoclonal antibody to PCNA on HeLa cell.

Detection limit for recombinant GST tagged PCNA is approximately 0.

ELISA

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.

Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PCNA MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (54.

7 images

1 reference

Catalog No. ABIN562135

$520.15

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 11 to 14 Business Days

Details

PCNA antibody (AA 1-261)

PCNA Reactivity: Human WB, ELISA Host: Mouse Monoclonal S1 unconjugated

PCNA monoclonal antibody (M04), clone S1 Western Blot analysis of PCNA expression in Hela S3 NE .

ELISA

PCNA monoclonal antibody (M04), clone S1.

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M04), clone S1.

7 images

1 reference

Catalog No. ABIN562136

$520.15

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 11 to 14 Business Days

Details

PCNA antibody

PCNA Reactivity: Human WB, IHC, IF, IP, IHC (p), ICC, IHC (fro), FISH Host: Rabbit Polyclonal unconjugated

The effects of ustekinumab and CUC on PCNA IE in IQ-induced psoriatic skin lesions.

ISt

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PCNA, antibody (Green) at 1:500 dilution.

ICC/IF Image PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.

IHC

IP Image Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody, Western blot analysis was performed using PCBP2 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis.

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.

IHC

Lycogen™ reduced cisplatin-induced cell apoptosis.

Combination of bortezomib and citreoviridin inhibited cell cycle progression through different cell cycle regulators.

Thyroid hormone induced proliferation in ovarian cancer cellsOVCAR-3 and SKOV-3 were treated with T3 (10-9 to 10-7 M) or T4 (10-8 to 10-6 M) for 3 days (A), 24 hours (B, upper panel) or 30 min (B, lower panel).

Changes in important mediators of inflammation and fibrosis in the obstructed kidney 7 days after UUO and the effects of Sirt1 activation or inhibition.

Prostate tumors grown from Cav1-silenced PC3 cells were accompanied by a more reactive tumor stroma.

IHC (p)

16 images

4 references

Catalog No. ABIN2854788

$564.19

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 5 Business Days

Details

PCNA antibody (C-Term)

PCNA Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17486 unconjugated

PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

IHC (p)

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) (ABIN389344 and ABIN2839453).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) (ABIN389344 and ABIN2839453) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

6 images

5 references

Catalog No. ABIN389344

$451.00

Plus shipping costs $50.00

400 μL (More quantities available)

Shipping to: United States

Delivery in 10 to 12 Business Days

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Fetuin A antibody (AA 200-240) (AbBy Fluor® 594)

AHSG Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal AbBy Fluor® 594

Immunohistological staining of artery tissues from diabetic subjects showing colocalization of serum proteins and mineral deposits.

IHC (p)

8 images

1 reference

Catalog No. ABIN2809553

$458.46

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 16 to 20 Business Days

Details

GAPDH antibody (AA 226-335)

GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431 .

GAPDH monoclonal antibody (M01), clone 3C2.

Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.

Detection limit for recombinant GST tagged GAPDH is 0.

ELISA

Immunoprecipitation of GAPDH transfected lysate using anti-GAPDH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GAPDH MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (37.

9 images

9 references

Catalog No. ABIN560997

$489.14

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 11 to 14 Business Days

Details

GAPDH antibody (Internal Region)

Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver.

ABIN185543 (2µg/ml) staining of paraffin embedded Human Liver.

IHC

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

11 images

6 references

Catalog No. ABIN185543

$488.25

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

beta Actin antibody

ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) antibody (ABIN962807)

Lane 1: 20 µg Hela cell lysate Lane 2: 20 µg sp2/0 cell lysate Lane 3: 20 µg goat muscle lysate Lane 4: 20 µg rabbit muscle lysate Lane 5: 20 µg chicken muscle lysate Lane 6: 20 µg CHO cell lysate Lane 7: 20 µg fish muscle lysate Primary antibody: 1 µg/mL Anti-beta-actin Monoclonal Antibody (Mouse) (ABIN396859) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (ABIN398387, 1: 20,000)

4 images

2 references

1 validation

Catalog No. ABIN962807

$323.08

Plus shipping costs $50.00

100 μg (More quantities available)

Shipping to: United States

Delivery in 6 to 9 Business Days

Details

Independently Validated

c-MYC antibody (AA 31-80)

MYC Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Myc Proto-Oncogene protein (MYC) (AA 31-80) antibody (ABIN1532205)

Immunocytochemistry validation image for anti-Myc Proto-Oncogene protein (MYC) (AA 31-80) antibody (ABIN1532205)

ICC

Western blot analysis of extracts from HeLa cells, treated with Forskolin 40nM 30', using MYC (Ab-62) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYC (Ab-62) Antibody.

IHC

Immunofluorescence analysis of HeLa cells, using MYC (Ab-62) Antibody.

5 images

2 validations

Catalog No. ABIN1532205

$341.00

Plus shipping costs $50.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 2 to 4 Business Days

Details

Independently Validated

MFN1 antibody (AA 1-234)

MFN1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Mitofusin 1 (MFN1) (AA 1-234) antibody (ABIN5013951)

IHC

5 images

1 validation

Catalog No. ABIN5013951

$252.00

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 15 to 19 Business Days

Details

Independently Validated

JAG1 antibody (N-Term)

Rockland 200-401-698 JAG1 Reactivity: Human, Mouse WB, IHC, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Jagged 1 (JAG1) (N-Term) antibody (ABIN1043767)

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody.

IHC

Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.

4 images

2 references

1 validation

Catalog No. ABIN129524

$564.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

500 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

Independently Validated

beta Catenin antibody (C-Term)

Rockland 600-401-c68 CATNB Reactivity: Human, Zebrafish (Danio rerio) WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Catenin, beta (CATNB) (C-Term) antibody (ABIN1043907)

Western Blot of Rabbit anti-Beta Catenin antibody.

Western Blot of Rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA Lane 3: zebrafish embryos un-injected Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT Predicted/Observed size: 85.

Immunohistochemistry of Rabbit anti-beta Catenin antibody.

IHC

4 images

2 references

1 validation

Catalog No. ABIN1043906

$509.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

100 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

Independently Validated

NPM1 antibody (C-Term)

NPM1 Reactivity: Human WB, IF, FACS, IHC (p), IP Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Immunofluorescence validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Flow Cytometry validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

FACS

Image no. 2 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Image no. 1 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

5 images

3 validations

Catalog No. ABIN1737584

$805.57

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 6 to 9 Business Days

Details

Independently Validated

SOD1 antibody (AA 2-154)

SOD1 Reactivity: Human, Horse WB, IHC, IP, IF/ICC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

Immunocytochemistry validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

ICC

Western blot analysis of recombinant Horse SOD1.

Western blot analysis of (1) Rat Serum, (2) Bovine Liver Tissue, (3) Bovine Heart Tissue, (4) Bovine Brain Tissue and (5) Bovine Serum.

4 images

1 reference

2 validations

Catalog No. ABIN2913314

$488.06

Plus shipping costs $50.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 10 to 20 Business Days

Details

Independently Validated

ODF2 antibody

ODF2 Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Immunocytochemistry validation image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430582)

ICC

Western Blotting (WB) image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430583)

Western blot analysis of Human testis tissue and K562 cell, using ODF2 Polyclonal Antibody at dilution of 1:650

4 images

2 references

2 validations

Catalog No. ABIN2430583

$538.80

Plus shipping costs $50.00

200 μL (More quantities available)

Shipping to: United States

Delivery in 12 to 15 Business Days

Details

Independently Validated

Recombinant RAD21 antibody (N-Term)

RAD21 Reactivity: Human, Mouse WB, IF Host: Mouse Monoclonal unconjugated Recombinant Antibody

Cleavage Under Targets and Release Using Nuclease validation image for anti-RAD21 Homolog (RAD21) (N-Term) antibody (ABIN6731038)

CUT&RUN

Rad21 antibody (rAb) tested by Western blot.

Rad21 antibody (rAb) tested by Immunofluorescence.

4 images

1 reference

1 validation

Catalog No. ABIN6731038

$530.77

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

Independently Validated

C10orf2 antibody (Middle Region)

C10ORF2 Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (Middle Region) antibody (ABIN2775251)

Immunocytochemistry validation image for anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (Middle Region) antibody (ABIN2775251)

ICC

WB Suggested Anti-PEO1 Antibody Titration: 0.

Host: Rabbit Target Name: PEO1 Sample Type: Hela Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5.

4 images

2 references

2 validations

Catalog No. ABIN2775251

$483.54

Plus shipping costs $50.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 2 to 4 Business Days

Details

Independently Validated

NME2 antibody (C-Term)

NME2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ICC, FACS Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152), (C-Term) antibody (ABIN3044198)

5 images

1 validation

Catalog No. ABIN3044198

$370.00

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 4 to 7 Business Days

Details

Independently Validated

MCL-1 antibody (Internal Region)

Rockland 600-401-394s MCL1 Reactivity: Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

Characterization of clonal lymphoid lines mutant for combinations of pro-apoptotic BCL2 family proteins.

ELISA

Rabbit anti-Mcl-1 WB Western Blot of Rabbit anti-Mcl-1 antibody.

4 images

86 references

Catalog No. ABIN6657787

$125.00

Plus shipping costs $50.00, if applicable $20.00 dry ice

25 μL

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

Rockland Original

Histone 3 antibody (H3K27me3)

H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

Histone H3 trimethyl Lys27 antibody tested by Western blot.

ChIP-seq

Histone H3 trimethyl Lys27 antibody specificity tested by peptide array analysis.

ChIP

Histone H3 trimethyl Lys27 antibody tested by immunofluorescence.

Histone H3 trimethyl Lys27 antibody tested by dot blot analysis.

5 images

39 references

Catalog No. ABIN2668416

$690.77

Plus shipping costs $50.00

100 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

Histone 3 antibody (H3K9me3)

H3 Reactivity: Human, Schizosaccharomyces pombe WB, IF, ChIP, ICC, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

Histone H3K9me3 antibody (pAb) tested by Western blot.

Dot blot of Histone H3 trimethyl Lys9 antibody.

Histone H3 trimethyl Lys9 antibody specificity tested by peptide array analysis.

PAr

Histone H3 trimethyl Lys9 antibody tested by ChIP-chip.

ChIP-seq

Histone H3 trimethyl Lys9 antibody tested by immunofluorescence.

5 images

33 references

Catalog No. ABIN2668470

$690.77

Plus shipping costs $50.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 1 to 3 Business Days

Details

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