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Western Blot Antibodies

Western blot (WB) antibodies are optimized for the detection of denatured proteins and validated to deliver clear, specific bands at the expected molecular weight. Use the filters below to quickly identify primary antibodies matched to your target, species, and experimental requirements.

Western Blot Antibody Validation

Antibodies for Western blot must reliably detect target proteins under denaturing conditions. Products in this category are validated for WB applications and selected for specific band detection at the expected molecular weight. Where available, validation data include experimental images, independent testing, and literature references to support antibody performance.

Western Blot Considerations

Protein denaturation during Western blotting can alter epitope structure, making antibody selection critical. Antibodies validated for WB are optimized to recognize linearized epitopes and reduce non-specific binding.

Monoclonal antibodies provide high specificity and consistent results, while polyclonal antibodies can offer stronger signal due to multi-epitope recognition. Recombinant antibodies combine defined specificity with improved reproducibility across experiments.

Western Blotting: Principles, Protocols, and Troubleshooting

Learn more about Western blotting, including protocols and troubleshooting

Use the filters below to narrow down Western blot antibodies by target, species, and experimental requirements.

529,062 results

Rockland 600-101-196 APOA1 Reactivity: Mouse WB, IHC, ELISA, IP Host: Goat Polyclonal unconjugated
Pubmed 10 references
Catalog No. ABIN5596808
$1,366.00
Plus shipping costs $50.00
1 mg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Rockland 600-401-919 SMAD3 Reactivity: Human WB, IHC, ELISA, FACS Host: Rabbit Polyclonal unconjugated
Pubmed 18 references
Catalog No. ABIN129675
$509.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Rockland 600-401-965 PDCD4 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Pubmed 11 references
Catalog No. ABIN129709
$509.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Rockland 200-301-379 RFP Reactivity: Discosoma WB, ELISA, IP, FACS, FM Host: Mouse Monoclonal 8E5-G7 unconjugated
Pubmed 16 references
Catalog No. ABIN1607680
$495.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
TFAM Reactivity: Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse, Pig WB Host: Rabbit Polyclonal unconjugated
Pubmed 10 references
Catalog No. ABIN2777277
$483.54
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 2 to 4 Business Days
 
HES1 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Horse WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
Pubmed 13 references
Catalog No. ABIN2779597
$429.69
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 2 to 4 Business Days
 
SARS-CoV-2 S Reactivity: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC Host: Rabbit Polyclonal unconjugated
Pubmed 11 references
Catalog No. ABIN1030641
$460.00
Plus shipping costs $50.00
0.1 mg
Shipping to: United States
Delivery in 2 to 4 Business Days
 
GFAP Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Pubmed 83 references
Catalog No. ABIN3043832
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
HIF1A Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Pubmed 33 references
Catalog No. ABIN3043841
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
LMNB1 Reactivity: Human, Mouse, Rat WB, IHC, IP, ICC, IF, FACS Host: Rabbit Polyclonal unconjugated
Pubmed 15 references
Catalog No. ABIN3042505
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
PECAM1 Reactivity: Human FACS, WB, IHC Host: Rabbit Polyclonal unconjugated
Pubmed 38 references
Catalog No. ABIN3043601
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
CD44 Reactivity: Human, Mouse, Rat FACS, WB, IHC, IF, ELISA, IHC (fro), ICC Host: Rabbit Polyclonal unconjugated
Pubmed 11 references
Catalog No. ABIN5692819
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
PECAM1 Reactivity: Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
Pubmed 16 references
Catalog No. ABIN5692990
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
DES Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Pubmed 22 references
Catalog No. ABIN3043265
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
VWF Reactivity: Rat, Mouse IHC, WB, IF Host: Rabbit Polyclonal unconjugated
Pubmed 17 references
Catalog No. ABIN3042349
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
CYCS Reactivity: Human, Rat, Mouse WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Pubmed 20 references
Catalog No. ABIN3042783
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
HSPA1A Reactivity: Human, Mouse, Rat WB, IHC, IF, ICC, FACS Host: Rabbit Polyclonal unconjugated
Pubmed 13 references
Catalog No. ABIN3043849
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
MYD88 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC, FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated
Pubmed 21 references
Catalog No. ABIN3043887
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
NFKB1 Reactivity: Human WB, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated
Pubmed 12 references
Catalog No. ABIN3043587
$370.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days
 
Reactivity: Human WB, IF, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Independent Validation 1 validation
Catalog No. ABIN7251292
$538.80
Plus shipping costs $50.00
200 μL (More quantities available)
Shipping to: United States
Delivery in 11 to 15 Business Days
Independently Validated
Reactivity: Plant WB Host: Mouse Monoclonal Q30 unconjugated
Pubmed 1 reference Independent Validation 1 validation
Catalog No. ABIN3181106
$402.79
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 11 to 15 Business Days
Independently Validated
HOXA13 Reactivity: Human WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Independent Validation 1 validation
Catalog No. ABIN1386749
$384.62
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 4 to 7 Business Days
Independently Validated
AQP3 Reactivity: Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Independent Validation 1 validation
Catalog No. ABIN863208
$548.75
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 3 to 5 Business Days
Independently Validated
PSA Reactivity: Human ELISA, WB, FACS, DB Host: Mouse Monoclonal A5D5-1 unconjugated
Independent Validation 1 validation
Catalog No. ABIN1543584
$839.81
Plus shipping costs $50.00
1 mg
Shipping to: United States
Delivery in 13 to 18 Business Days
Independently Validated
MYO1F Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Independent Validation 2 validations
Catalog No. ABIN7429520
$326.15
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 13 to 17 Business Days
Independently Validated
  • Type Primary
    • Primary
  • Target
    • Histone 3 (H3)
    • p53 (TP53)
    • EGFR
    • tau
    • AKT1
    • NF-kB p65 (NFkBP65)
    • Estrogen Receptor alpha (ESR1)
    • Bcl-2 (BCL2)
    • KIT
    • TNF alpha
    • RAF1
    • c-MYC (MYC)
    • FAK (PTK2)
    • Vimentin (VIM)
    • GFAP
    • RPS6KB1
    • HSP27 (HSPB1)
    • ErbB2/Her2
    • Cytokeratin 18 (KRT18)
    • HSP70
    • Albumin (ALB)
    • CDK1
    • C-JUN (JUN)
    • IL-6 (IL6)
    • Histone H4 (HISTH4)
    • ATF2
    • STAT3
    • EpCAM (EPCAM)
    • SMAD2
    • Caspase 9 (CASP9)
    • GAPDH
    • SNCA
    • Cytokeratin 19 (KRT19)
    • MEK1 (MAP2K1)
    • Interferon gamma (IFNG)
    • beta Catenin (CATNB)
    • E-cadherin (CDH1)
    • Caspase 3 (CASP3)
    • CREB1
    • HSPD1
    • CD31 (PECAM1)
    • Histone H2B
    • SOD1
    • BAD
    • GRP78 (HSPA5)
    • ERK1 (MAPK3)
    • Src
    • CD45 (PTPRC)
    • eIF4EBP1 (EIF4EBP1)
    • FGFR1
    Please use search to find more...
  • Application Western Blotting (WB)
    • Western Blotting (WB)
    • ELISA
    • Immunohistochemistry (IHC)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunofluorescence (IF)
    • Flow Cytometry (FACS)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunoprecipitation (IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Enzyme Immunoassay (EIA)
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunofluorescence (fixed cells) (IF/ICC)
    • Dot Blot (DB)
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Immunochromatography (IC)
    • Functional Studies (Func)
    • Radioimmunoassay (RIA)
    • Neutralization (Neut)
    • Chromatin Immunoprecipitation (ChIP)
    • Coating (Coat)
    • Staining Methods (StM)
    • Immunostaining (ISt)
    • Fluorescence Microscopy (FM)
    • Immunoassay (IA)
    • Immunodiffusion (ID)
    • Proximity Ligation Assay (PLA)
    • Lateral Flow (LF)
    • Surface Plasmon Resonance (SPR)
    • Confocal Microscopy (CM)
    • Immunoelectrophoresis (IEP)
    • Live Cell Imaging (LCI)
    • Bio-Layer Interferometry (BLI)
    • Cytotoxicity Test (CyTox)
    • Indirect Immunofluorescence Assay (IFA)
    • RNA Interference (RNAi)
    • Antibody Array (AA)
    • Blocking Antibody (Inhibition)
    • Blocking Reagent (BR)
    • Blocking Peptide (BP)
    • ELISA (Capture)
    • Chemiluminescence Immunoassay (CLIA)
    • ChIP DNA-Sequencing (ChIP-seq)
    • In vivo Studies (in vivo)
    • Protein Assay (PrA)
    • Affinity Purification (AP)
    • Cellular Assay (CA)
    • Intracellular Flow Cytometry (ICFC)
    • Luminex Assay (LMNX)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Cow
    • Dog
    • Rabbit
    • Horse
    • Guinea Pig
    • Pig
    • Monkey
    • Zebrafish (Danio rerio)
    • Chicken
    • Bat
    • Sheep
    • Hamster
    • Xenopus laevis
    • Goat
    • Saccharomyces cerevisiae
    • Drosophila melanogaster
    • Arabidopsis thaliana
    • Virus
    • E. coli
    • Cat
    • Various Species
    • Plant
    • Human Immunodeficiency Virus (HIV)
    • Mammalian
    • Frog
    • Fish
    • Bacteria
    • Hepatitis C Virus (HCV)
    • Aequorea victoria
    • Oryza sativa
    • Chimpanzee
    • Non-Human Primate
    • Zea mays
    • Primate
    • C. elegans
    • Rhesus Monkey
    • SARS Coronavirus-2 (SARS-CoV-2)
    • Tag
    • Chemical
    • Chlamydomonas reinhardtii
    • Cynomolgus
    • Avian
    • Barley
    • Nicotiana tabacum
    • Arabidopsis
    • Influenza A Virus
    • Hepatitis B Virus (HBV)
    Please use search to find more...
  • Cited in publications
  • Images available
  • Independent Validation
  • Preservative free only
  • Carrier free only
  • Detection Method
    • Fluorometric
  • Host
    • Rabbit
    • Mouse
    • Goat
    • Sheep
    • Rat
    • Chicken
    • Guinea Pig
    • Human
    • Hamster
    • Alpaca
    • Mammalian Cells
    • Humanized
    • Chicken egg
    • Llama
    • Cell culture
    • Human Cells
    • Armenian Hamster
    • Cow
    • HEK-293F Cells
    • Chicken eggs
    • Golden Syrian Hamster
    • Horse
    • CHO Cells
    • Dog
    • Donkey
    • Carp
    • Fruit Fly (Drosophila melanogaster)
    • Phage Display
    • Pig
    • Tobacco (Nicotiana benthamiana)
  • Clonality
    • Polyclonal
    • Monoclonal
    • Chimeric
    • Bispecific
  • Clone
    • C1
    • C2
    • C3
    • C4
    • C5
    • C6
    • C10
    • C7
    • C11
    • D1
    • D4
    • C8
    • 1F6
    • 1D6
    • C12
    • D2
    • 2C4
    • 2B11
    • D3
    • 1F4
    • C9
    • 1C4
    • 1D2
    • 3G1
    • 1F10
    • 2D3
    • 1F3
    • 2E9
    • 2F11
    • 1A8
    • 1F11
    • 1A7
    • 1D4
    • 1E8
    • 1F8
    • 1G2
    • 1G5
    • 3F8
    • 3G4
    • 1A5
    • 1B2
    • 1D8
    • 1C1
    • 1E1
    • 2C1
    • 1A4
    • 2D7
    • 1A1
    • 1B4
    • 1B7
    Please use search to find more...
  • Binding Specificity
    • C-Term
    • N-Term
    • Center
    • Internal Region
    • Middle Region
    • AA 101-200
    • AA 1-100
    • AA 1-30
    • AA 51-150
    • AA 201-300
    • AA 151-250
    • AA 21-120
    • AA 301-400
    • AA 251-350
    • AA 1-50
    • AA 31-130
    • AA 401-500
    • AA 351-450
    • AA 121-220
    • AA 501-600
    • AA 131-230
    • AA 451-550
    • Extracellular
    • AA 1-80
    • AA 81-180
    • AA 61-160
    • AA 231-330
    • Extracellular Domain
    • AA 221-320
    • AA 41-140
    • AA 35-84
    • AA 71-120
    • AA 601-700
    • AA 161-260
    • Intracellular
    • AA 331-430
    • AA 51-100
    • AA 50-100
    • AA 181-280
    • AA 701-800
    • Cytosolic
    • AA 551-650
    • AA 1-300
    • AA 141-240
    • AA 1-110
    • AA 30-110
    • AA 1-200
    • AA 107-156
    • AA 251-300
    • AA 65-160
    Please use search to find more...
  • Conjugate
    • Un-conjugated
    • Biotin
    • FITC
    • HRP
    • PE
    • APC
    • Cy3
    • AbBy Fluor® 594
    • Cy5
    • AbBy Fluor® 350
    • AbBy Fluor® 488
    • AbBy Fluor® 555
    • AbBy Fluor® 647
    • Cy5.5
    • Cy7
    • AbBy Fluor® 750
    • AbBy Fluor® 680
    • Alexa Fluor 350
    • Alexa Fluor 405
    • Alexa Fluor 647
    • Alexa Fluor 750
    • Alexa Fluor 488
    • Alexa Fluor 594
    • Alexa Fluor 555
    • Alexa Fluor 680
    • CF®740
    • CF®488A
    • CF®405S
    • CF®568
    • CF®640R
    • CF®647
    • CF®594
    • PE-Cy5
    • PE-Cy7
    • PE-Cy5.5
    • PerCP
    • Atto 488
    • Atto 390
    • Atto 594
    • Alkaline Phosphatase (AP)
    • Peroxidase (POD)
    • DyLight 488
    • Texas Red (TR)
    • DyLight 680
    • DyLight 800
    • DyLight 405
    • DyLight 549
    • DyLight 649
    • CF®555
    • Rhodamine
    • His tag
  • Isotype
    • IgG
    • Ig Fraction
    • IgG1
    • IgG2a
    • IgG1 kappa
    • IgG2b
    • kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG2
    • IgM
    • IgY
    • IgG3
    • IgG kappa
    • IgM kappa
    • IgG3 kappa
    • IgG2c
    • IgG1 lambda
    • IgG Mix lambda
    • IgG2a lambda
    • IgG2c kappa
    • L234A
    • L235A
    • P329G
    • IgG2b lambda
    • IgG lambda
    • IgG4
    • IgG2 lambda
    • Ig kappa
    • IgG1/IgG2b
    • IgG2 kappa
    • lambda
    • IgA
    • IgA kappa
    • IgG Mix kappa
    • D265A
    • IgG1b kappa
    • IgG3 lambda
    • IgG IgA IgM Mix lambda
    • IgG1a
    • IgG4 kappa
    • IgM lambda
    • Ig
    • Ig Mix
    • IgG2c lambda
    • Fc Silenced
    • IgA2 kappa
    • IgE
    • IgE kappa
    • IgG IgA IgM Mix
    Please use search to find more...
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
    • Frozen
    • Lyophilized or Liquid
  • Fragment
    • single-domain Antibody (sdAb)
    • Fc fragment
    • Fab fragment
    • scFv fragment
    • F(ab')2 fragment
  • Grade
    • Verified
    • KD Validated
    • KO Validated
    • Carrier-free
    • Cell Culture Grade
    • In vivo Grade
    • KO Validated, KD Validated
    • Research Grade
    • ChIP-seq Grade
    • Animal-Free
    • GMP Grade
    • ChIP Grade
    • Calibrator Grade
    • MALS verified
    • Recent
  • Supplier
    • antibodies-online
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    • Aviva Systems Biology
    • Abbexa
    • Acris
    • Boster Bio
    • ProSci
    • Biotium
    • Assay Biotechnology Company, Inc.
    • Biomatik
    • Cloud-Clone
    • NSJ Bioreagents
    • FabGennix
    • RayBiotech
    • Rockland
    • Covalab
    • Angio-Proteomie
    • CUSABIO
    • Agrisera
    • Alomone Labs
    • Enogene Biotech
    • Antibodies Incorporated
    • Abiocode
    • OriGene
    • Wuhan Fine biotech
    • Elabscience
    • Synaptic Systems
    • Abeomics
    • RevMAb Biosciences
    • Active Motif
    • QED Bioscience
    • Immune Technology
    • ProteoGenix
    • PROGEN
    • Kamiya Biomedicals
    • Biospes
    • EnCor Biotechnology
    • BioAcademia
    • Hytest
    • Diagenode
    • Neuromics Antibodies
    • ANTIGENES
    • AntiProt
    • Assaypro
    • Virusys
    • GenScript
    • Anogen
    • Torrey Pines
    Please use search to find more...
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