anti-Human FASL antibody for Western Blotting

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • FASL
  • TNFSF6
  • fasl
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • FASLG
  • zgc:162027
  • Fas ligand
  • Fas ligand L homeolog
  • Fas ligand (TNF superfamily, member 6)
  • FASLG
  • faslg.L
  • Fasl
  • Faslg
  • faslg
Epitope
AA 263-281, C-Term
25
18
17
16
13
12
9
7
7
6
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
438
176
87
36
7
5
5
3
3
2
1
Host
Rabbit
278
171
49
33
32
9
1
Clonality
Polyclonal
Conjugate
This FASL antibody is un-conjugated
47
32
29
14
13
13
10
10
9
9
9
9
9
9
8
7
7
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
338
261
127
100
76
70
52
48
29
28
13
12
10
10
9
5
3
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN3043159
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2908103 ABIN2779355 WB Rabbit Middle Region Log in to see Polyclonal 0
3.2908103 ABIN2705755 WB Rabbit Center Log in to see Polyclonal 0
3.2908103 ABIN967520 IHC (fro) FACS IP WB Mouse IgG1 Log in to see G247-4 7
3.2908103 ABIN1533270 IHC ELISA WB Rabbit IgG AA 101-150 Log in to see Polyclonal 0
3.2908103 ABIN4271438 CyTOF ELISA FACS WB Mouse IgG1 Log in to see 4F8D6 2
3.2908103 ABIN4310549 ELISA WB Goat Log in to see Polyclonal 1
3.2908103 ABIN1858802 ICC IHC WB Rabbit IgG AA 103-281 Log in to see Polyclonal 0
3.2908103 ABIN223056 IP IHC WB Mouse IgG1 Log in to see I-6060 1
3.2908103 ABIN3184595 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.2908103 ABIN155064 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see I-6060 0
3.2908103 ABIN1872660 WB Rabbit IgG Log in to see Polyclonal 0
3.2908103 ABIN4271439 WB Rabbit IgG Center Log in to see Polyclonal 0
3.2908103 ABIN1176953 FACS IP Neut ELISA WB Mouse IgG2a Log in to see NOK-2 3
3.2908103 ABIN4310542 IHC IP WB Mouse IgG1 Log in to see I-6060 0
3.2908103 ABIN615525 IHC (p) WB Rabbit Log in to see Polyclonal 0
3.2908103 ABIN1172027 ICC IHC WB Rabbit IgG AA 103-281 Log in to see Polyclonal 0
3.2908103 ABIN4271399 FACS WB Rat IgG2a AA 103-281 Log in to see Mike-2 0
3.2908103 ABIN4310550 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F8H6 0
3.2908103 ABIN4271498 WB Mouse IgG1 Log in to see 1F2 0
3.2908103 ABIN4310547 IP WB Rabbit AA 285-335 Log in to see Polyclonal 0

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope AA 263-281, C-Term
(25), (18), (17), (16), (13), (12), (9), (7), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(438), (176), (87), (36), (7), (5), (5), (3), (3), (2), (1)
Host Rabbit
(278), (171), (49), (33), (32), (9), (1)
Clonality
Polyclonal
Conjugate This FASL antibody is un-conjugated
(47), (32), (29), (14), (13), (13), (10), (10), (9), (9), (9), (9), (9), (9), (8), (7), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(338), (261), (127), (100), (76), (70), (52), (48), (29), (28), (13), (12), (10), (10), (9), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN3043159) Images
Purpose Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.
Sequence ELSLVNFEES QTFFGLYKL
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.
Gene Name: Fas ligand(TNF superfamily, member 6)
Protein Name: Tumor necrosis factor ligand superfamily member 6
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(263-281aa ELSLVNFEESQTFFGLYKL).
Isotype IgG

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN3043159) Images back to top
Antigen
Alternative Name FASLG (FASL Antibody Abstract)
Background Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Synonyms: ALPS1B antibody|APO1L antibody|Apoptosis(APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|FAS antigen ligand antibody|Fas L antibody|Fas ligand(TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand(TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNF superfamily member 6 antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor(ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody
UniProt P48023
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN3043159) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN3043159) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-FASL antibody (ABIN3043159)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Product cited in:

Liu, Su, Zhang, Zhang, Wang, Qian: "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 386-98, 2015

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, 2014

Ma, Zhang, Zhang, Yang, Meng, Zhang, Zhou: "The effect of esculentoside A on lupus nephritis-prone BXSB mice." in: Archives of medical science : AMS, Vol. 9, Issue 2, pp. 354-60, 2013

Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, 2010

Wang, Zhang, Lian: "Aberrant expression of Fas and FasL pro-apoptotic proteins in basal cell and squamous cell carcinomas." in: Clinical and experimental dermatology, Vol. 36, Issue 1, pp. 69-76, 2010

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, 2009

Wang, Tang, Yang, Yao, Chen, Liu, Li: "The expression of Fas ligand on normal and stabbed-disc cells in a rabbit model of intervertebral disc degeneration: a possible pathogenesis." in: Journal of neurosurgery. Spine, Vol. 6, Issue 5, pp. 425-30, 2007

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN3043159) back to top
Supplier Images
Western Blotting (WB) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 263-281), (C-Term) antibody (ABIN3043159) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 263-281), (C-Term) antibody