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|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Epitope||AA 263-281, C-Term Alternatives|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|7 references available|
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Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling References for anti-FASL antibody (ABIN3043159) Images
|Purpose||Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.
Gene Name: Fas ligand(TNF superfamily, member 6)
Protein Name: Tumor necrosis factor ligand superfamily member 6
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(263-281aa ELSLVNFEESQTFFGLYKL).|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN3043159) Images back to top
|Alternative Name||FASLG (FASL Antibody Abstract)|
Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Synonyms: ALPS1B antibody|APO1L antibody|Apoptosis(APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|FAS antigen ligand antibody|Fas L antibody|Fas ligand(TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand(TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNF superfamily member 6 antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor(ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody
|Pathways||Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN3043159) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN3043159) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
References for anti-FASL antibody (ABIN3043159)Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
|Product cited in:||
Liu, Su, Zhang, Zhang, Wang, Qian: "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 386-98, 2015
Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, 2014
Ma, Zhang, Zhang, Yang, Meng, Zhang, Zhou: "The effect of esculentoside A on lupus nephritis-prone BXSB mice." in: Archives of medical science : AMS, Vol. 9, Issue 2, pp. 354-60, 2013
Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, 2010
Wang, Zhang, Lian: "Aberrant expression of Fas and FasL pro-apoptotic proteins in basal cell and squamous cell carcinomas." in: Clinical and experimental dermatology, Vol. 36, Issue 1, pp. 69-76, 2010
Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, 2009
Wang, Tang, Yang, Yao, Chen, Liu, Li: "The expression of Fas ligand on normal and stabbed-disc cells in a rabbit model of intervertebral disc degeneration: a possible pathogenesis." in: Journal of neurosurgery. Spine, Vol. 6, Issue 5, pp. 425-30, 2007
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN3043159) back to top