anti-Dog (Canine) YWHAQ antibody for Western Blotting

Recommended YWHAQ Antibody (supplied by: Log in to see )

Antigen
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
  • 14-3-3
  • 14-3-3t
  • 1c5
  • hs1
  • 1C5
  • HS1
  • 2700028P07Rik
  • AA409740
  • AU021156
  • R74690
  • ywhaq
  • ywhaq2
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
  • 14-3-3 protein theta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a
  • ywhaq
  • YWHAQ
  • Ywhaq
  • ywhaq.S
  • ywhaqa
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
179
121
108
40
25
24
17
14
14
12
10
10
6
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
Host
Mouse
129
37
17
7
1
Clonality
Monoclonal
Conjugate
This YWHAQ antibody is un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
162
59
50
39
34
26
24
8
5
4
3
3
1
1
Options
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Catalog No. ABIN2688836
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.280777 ABIN2792144 IHC WB Rabbit N-Term Log in to see Polyclonal 2
3.280777 ABIN2463667 IHC ELISA WB Rabbit Log in to see Polyclonal 0
3.280777 ABIN4276649 SimWes WB Rabbit Log in to see Polyclonal 0
3.280777 ABIN926887 IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.280777 ABIN2559737 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN5553400 WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN296621 ELISA WB Goat AA 136-146 Log in to see Polyclonal 0
1 ABIN2737987 WB Mouse IgG Log in to see 0
1 ABIN609109 IHC (p) ELISA WB Rabbit IgG AA 1-10 Log in to see Polyclonal 0
1 ABIN2629936 IHC (p) WB Rabbit IgG AA 45-94 Log in to see Polyclonal 0
1 ABIN304752 WB Sheep pSer185 Log in to see Polyclonal 0
1 ABIN1099738 IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN4276648 SimWes WB Rabbit Log in to see Polyclonal 0
1 ABIN1949261 IF IP IHC (p) WB Rabbit IgG AA 195-245 Log in to see Polyclonal 0
1 ABIN1731306 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN393005 WB Rabbit pSer58 Log in to see Polyclonal 0
1 ABIN331518 ICC IHC WB Mouse IgG Log in to see 22-IID8B 0
1 ABIN5688864 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5688906 IHC WB Rabbit pSer58 Log in to see Polyclonal 0
1 ABIN1582184 IHC ELISA WB Rabbit pSer58 Log in to see Polyclonal 0

General

Antigen tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(179), (121), (108), (40), (25), (24), (17), (14), (14), (12), (10), (10), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(129), (37), (17), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This YWHAQ antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(162), (59), (50), (39), (34), (26), (24), (8), (5), (4), (3), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-YWHAQ Antibody

Target Details YWHAQ Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) Images
Characteristics The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ. Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- 14-3-3 antibody. Immunofluorescence staining of SKN cells (Human neuroblastoma).

BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3 - Purified - Clone 12/14-3-3 - Isotype Mouse IgM - Reactivity Hu, Chick, Dog, Frog, Ms, Rat - 150 μg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human 14-3-3
Clone 12-14-3
Isotype IgM

Target Details YWHAQ

Product Details anti-YWHAQ Antibody Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Antigen
Alternative Name 14-3-3 (YWHAQ Antibody Abstract)
Molecular Weight 29 kDa
Pathways Apoptosis, Myometrial Relaxation and Contraction

Application Details

Product Details anti-YWHAQ Antibody Target Details YWHAQ Handling References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-YWHAQ antibody (ABIN2688836)

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling Images back to top
Product cited in:

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, 2002

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, 2001

Ostrerova, Petrucelli, Farrer, Mehta, Choi, Hardy, Wolozin: "alpha-Synuclein shares physical and functional homology with 14-3-3 proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5782-91, 1999

Conklin, Galaktionov, Beach: "14-3-3 proteins associate with cdc25 phosphatases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7892-6, 1995

Freed, Symons, Macdonald, McCormick, Ruggieri: "Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1713-6, 1994

Images

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) back to top
Supplier Images
Western Blotting (WB) image for anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) antibody (ABIN2688836) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) antibody