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Ponceau S solution

WB 1 X

Catalog No. ABIN5706409

Datasheet as PDF

Application

Western Blotting (WB)

Purification

Ponceau S Staining Solution contains Ponceau S in a specially formulated acetate buffer for optimal staining results. Ponceau S is a ready-to-use stain that can be used to quickly and reversible stain of proteins on blotting membranes after Western blot transfer. Staining of Nylon membranes is not recommended.
Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X

Revitablot™ Western Blot Stripping Buffer
WB

Fluoromount-G®
FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL

ProXtract B™ Extraction Buffer
WB

Blocking Buffer for Fluorescent Western Blotting (2X)
WB

Wash Solution (5X)
WB 5 X

ELISA Coating Stabilizer
ELISA 1 X

ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
ELISA 1 X

TMB Membrane Peroxidase Substrate (Ready-To-Use)
DB, WB 1 X

Transfer Buffer Powder
SDS

Application Notes

Application Note: Store at room temperature. The product is at working concentration, no further dilution is necessary. The limit of detection for this stain is 200 nanograms of protein after separation by electrophoresis in polyacrylamide gels and transferred to nitrocellulose or PVDF membranes. Ponceau S is not suitable for use with nylon membranes.
Western Blot Dilution: ready to use

Comment

Synonyms: Staining, Western Blot membrane stains, PVDF stains, nitrocellulose stain, reversible membrane stain, cellulose acetate membrane stain
Background: Ponceau S Staining Solution is made using Ponceau S. Ponceau S is a sodium salt used for the detection of proteins on PVDF, and nitrocellulose membranes. Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. Ponceau S binds rapidly and reversibly to the positively charged amino groups and binds non-covalently to non-polar regions in the protein. Ponceau S stain is designed for rapid (5 min) staining on nitrocellulose or PVDF membranes and also for staining protein on cellulose acetate membranes. Microgram quantities of transferred protein will be detected as red protein bands with a clear background. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with dH2O or blocking buffer, this allows further immunological detection.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 X

Buffer

Stabilizer: None

Preservative

Without preservative

Storage

RT

Expiry Date

6 months