10X TBS pH 7.5

Catalog No. ABIN925552

Product Details

Application: ELISA, Western Blotting (WB)

Purification: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Aseptic filtered

Application Details

Application Notes:

Application Note: This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris Buffered Saline consists of 1.0 M Tris HCl and 1.5 M Sodium Chloride,  pH 7.5, meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI).

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Comment:

Synonyms: TBS, Tris Buffered Saline

Background: Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 X

Buffer:

Buffer: See application note.

Stabilizer: None

Preservative: Without preservative

Storage: RT,4 °C

Expiry Date: 6 months