anti-Rat (Rattus) IL1B antibody for Radioimmunoassay

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Reactivity
Mouse (Murine), Rat (Rattus)
511
189
138
37
28
21
19
15
15
11
10
9
8
4
4
2
2
1
1
Host
Rabbit
433
331
17
10
10
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
102
45
32
15
14
11
10
9
9
8
8
8
8
8
8
8
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
547
378
215
132
102
73
61
56
44
40
33
32
23
15
14
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN964782
$ 389.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN964792 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN964790 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN5556655 EIA IF IHC (fro) IHC (p) IP Neut RIA WB Rabbit Log in to see Polyclonal 0

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(511), (189), (138), (37), (28), (21), (19), (15), (15), (11), (10), (9), (8), (4), (4), (2), (2), (1), (1)
Host Rabbit
(433), (331), (17), (10), (10), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(102), (45), (32), (15), (14), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(547), (378), (215), (132), (102), (73), (61), (56), (44), (40), (33), (32), (23), (15), (14), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW mouse IL-1ß and is useful in determining its presence in various assays. The antibody does not recognize human IL-1ß or mouse IL-1alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1ß. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured 31,000 precursor IL-1ß will be recognized by this antibody.
Cross-Reactivity Rat (Rattus)
Characteristics IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.
Purification purified
Immunogen This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli.  The MW of recombinant mouse IL-1ß was 17 kDa.
Immunogen Type: RecombinantProtein
Isotype IgG
Plasmids, Primers & others

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL1 beta (IL1B Antibody Abstract)
Background IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.
Synonyms: IL-1 beta, Interleukin-1 beta, IL-1ß, catabolin
Gene ID 16176
UniProt P10749
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Anti-Mouse IL-1ß has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots, typically, IL-1ß is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1ß. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1ß activity in bioassays. It does not neutralize the biological activity IL-1α. It does not neutralize the biological activity of human or primate IL-1ß. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Comment

Gene Name: Il1b

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
Expiry Date 6 months

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN964782) Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN964782) This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mo...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) antibody (ABIN964782) Immunohistochemistry of Rabbit anti-IL1Beta Antibody in Mouse Embryonic Kidney Tissue...