anti-Human CD28 antibody for Immunocytochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Reactivity
Human
493
414
101
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Goat
525
203
92
68
40
28
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
112
95
73
56
21
20
20
19
17
17
13
13
13
13
12
12
12
8
8
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
783
209
185
161
146
143
133
128
89
77
26
19
13
9
8
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Pubmed
4 references available

Available images

Immunocytochemistry (ICC) image for anti-CD28 (CD28) antibody (ABIN5012855)
Functional Studies (Func) image for anti-CD28 (CD28) antibody (ABIN5012855)
Catalog No. ABIN5012855
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN457388 FACS Func ICC IHC (f) IP WB PerCP Mouse IgG1 Log in to see CD28-2 6
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Golden Syrian Hamster IgG Log in to see 37-51 0
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN4291249 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75 0
1 ABIN4291251 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77 0
1 ABIN4291250 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77 0
1 ABIN4291254 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28 0
1 ABIN4291252 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77 0
1 ABIN4291253 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28 0
1 ABIN4291255 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28 0
1 ABIN4291247 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75 0
1 ABIN4291248 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75 0
1 ABIN1959966 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN1964045 ELISA ICC IF WB APC Rabbit IgG AA 182-208 Log in to see Polyclonal 0
1 ABIN1972544 ELISA ICC IF WB HRP Rabbit IgG AA 182-208 Log in to see Polyclonal 0

General

Antigen CD28 Antibodies
Reactivity Human
(493), (414), (101), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Goat
(525), (203), (92), (68), (40), (28), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(112), (95), (73), (56), (21), (20), (20), (19), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(783), (209), (185), (161), (146), (143), (133), (128), (89), (77), (26), (19), (13), (9), (8), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN5012855) Images
Specificity Detects human CD28 in direct ELISAs and Western blots. In direct ELISAs, approximately 20 % cross-reactivity with recombinant mouse CD28 is observed.
Purification Immunogen affinity purified
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN5012855) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Alternative Names: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 Molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene ID 940
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN5012855) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Agonist Activity 0.3-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN5012855) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD28 antibody (ABIN5012855)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Ellinghaus, Cortini, Pinder, Le Friec, Kemper, Vyse: "Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus." in: European journal of immunology, Vol. 47, Issue 7, pp. 1200-1210, 2017 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Boyette, Macedo, Hadi, Elinoff, Walters, Ramaswami, Chalasani, Taboas, Lakkis, Metes: "Phenotype, function, and differentiation potential of human monocyte subsets." in: PLoS ONE, Vol. 12, Issue 4, pp. e0176460, 2017 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Bahlis, King, Kolonias, Carlson, Liu, Hussein, Terebelo, Byrne, Levine, Boise, Lee: "CD28-mediated regulation of multiple myeloma cell proliferation and survival." in: Blood, Vol. 109, Issue 11, pp. 5002-10, 2007 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Sakthivel, Shively, Kakoulidou, Pearce, Lefvert: "The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm." in: Journal of internal medicine, Vol. 261, Issue 4, pp. 399-407, 2007 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN5012855) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-CD28 (CD28) antibody (ABIN5012855) CD28 in Human PBMCs. CD28 was detected in immersion fixed human peripheral blood mono...
Functional Studies (Func) image for anti-CD28 (CD28) antibody (ABIN5012855) Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 Antigen Affin...