anti-Mouse (Murine) COPA antibody for Western Blotting

Recommended COPA Antibody (supplied by: Log in to see )

Antigen
Coatomer Protein Complex, Subunit alpha (COPA) Antibodies
  • COPA
  • DDBDRAFT_0189693
  • DDBDRAFT_0233797
  • DDB_0189693
  • DDB_0233797
  • DKFZp469O221
  • AU040324
  • xenin
  • HEP-COP
  • cb281
  • fb13c12
  • wu:fb13c12
  • coatomer protein complex subunit alpha
  • WD40 repeat-containing protein
  • Coatomer subunit alpha
  • coatomer protein complex, subunit alpha
  • coatomer protein complex subunit alpha L homeolog
  • COPA
  • copA
  • cgd8_860
  • Chro.80105
  • AFUA_3G08840
  • copa
  • Copa
  • copa.L
Epitope
N-Term
3
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
24
9
7
6
6
5
5
5
5
5
4
4
4
4
4
1
Host
Rabbit
14
9
1
Clonality
Polyclonal
Conjugate
This COPA antibody is un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
23
15
3
2
1
1
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Catalog No. ABIN2784677
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.080771 ABIN2784678 WB Rabbit Middle Region Log in to see Polyclonal 1
3.080771 ABIN634777 WB Rabbit N-Term Log in to see Polyclonal 0
3.080771 ABIN634778 WB Rabbit Middle Region Log in to see Polyclonal 0
3.080771 ABIN2463478 ELISA WB Rabbit Log in to see Polyclonal 0
3.080771 ABIN2561950 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN321894 WB Rabbit IgG AA 22-71 Log in to see Polyclonal 0
1 ABIN337288 ICC IHC IHC (p) WB Rabbit IgG AA 1-19 Log in to see Polyclonal 0
1 ABIN469430 WB Rabbit AA 396-445 Log in to see Polyclonal 0
1 ABIN2740049 ICC IF WB Rabbit IgG AA 1-19 Log in to see Polyclonal 0

General

Antigen Coatomer Protein Complex, Subunit alpha (COPA) Antibodies
Epitope N-Term
(3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
(24), (9), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (1)
Host Rabbit
(14), (9), (1)
Clonality
Polyclonal
Conjugate This COPA antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(23), (15), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-COPA Antibody

Target Details COPA Application Details Handling References for anti-COPA antibody (ABIN2784677) Images
Blocking Peptide Blocking peptide for this product available: ABIN975184
Sequence PWILTSLHNG VIQLWDYRMC TLIDKFDEHD GPVRGIDFHK QQPLFVSGGD
Predicted Reactivity Cow: 100%, Dog: 100%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against COPA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human COPA
Plasmids, Primers & others

Target Details COPA

Product Details anti-COPA Antibody Application Details Handling References for anti-COPA antibody (ABIN2784677) Images back to top
Antigen
Alternative Name COPA (COPA Antibody Abstract)
Background In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone.In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms.
Alias Symbols: FLJ26320, HEP-COP
Protein Interaction Partner: UBC, SUMO2, SMN1, STAU1, NUDCD1, SUMO1, NEDD8, RPA3, RPA2, RPA1, SUZ12, RNF2, COPZ1, DHX38, COPB2, CCT3, TCP1, SEPT2, ARCN1, UBD, VCAM1, ITGA4, COPB1, COPG2, COPG1, COPE, SEPT9, SEC23A, SEC24C, UBXN6, FBXO6, LMAN1, SACM1L, DDX20, TMED10, GEMIN2, COPA, CDK
Protein Size: 1224
Molecular Weight 135 kDa
Gene ID 1314
NCBI Accession NM_004371, NP_004362
UniProt P53621
Pathways Hormone Activity

Application Details

Product Details anti-COPA Antibody Target Details COPA Handling References for anti-COPA antibody (ABIN2784677) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1224 AA

Restrictions For Research Use only

Handling

Product Details anti-COPA Antibody Target Details COPA Application Details References for anti-COPA antibody (ABIN2784677) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COPA antibody (ABIN2784677)

Product Details anti-COPA Antibody Target Details COPA Application Details Handling Images back to top
Product cited in:

Li, Custer, Gilson, Hao, Beattie, Androphy: "α-COP binding to the survival motor neuron protein SMN is required for neuronal process outgrowth." in: Human molecular genetics, Vol. 24, Issue 25, pp. 7295-307, 2015

Ting, Wen, Liu, Hsieh-Li, Li, Lin-Chao: "The spinal muscular atrophy disease protein SMN is linked to the Golgi network." in: PLoS ONE, Vol. 7, Issue 12, pp. e51826, 2013

Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-COPA Antibody Target Details COPA Application Details Handling References for anti-COPA antibody (ABIN2784677) back to top
Supplier Images
Western Blotting (WB) image for anti-Coatomer Protein Complex, Subunit alpha (COPA) (N-Term) antibody (ABIN2784677) WB Suggested Anti-COPA Antibody Titration: 0.2-1 ug/ml
Positive Control:...