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|Antigen||Protein Kinase C, delta (PKCd) Antibodies|
|Conjugate||This PKC delta antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Product Details anti-PKC delta AntibodyTarget Details PKC delta Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PKC delta protein. (region surrounding asn639)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purity||> 95 % by SDS-PAGE|
|Plasmids, Primers & others|
Target Details PKC deltaProduct Details anti-PKC delta Antibody Application Details Handling Images back to top
|Alternative Name||PRKCD (PKCd Antibody Abstract)|
|Background||Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol esterbinding activities and kinase activities.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type|
|Molecular Weight||approx. 80 kDa|
|Pathways||Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism|
Application DetailsProduct Details anti-PKC delta Antibody Target Details PKC delta Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling back to top