(1 reference)-
- Target See all IgG2b products
- IgG2b
-
Host
- Mouse
- Conjugate
- Alexa Fluor 647
- Application
- Isotype Control (IsoC), Intracellular Flow Cytometry (ICFC)
- Brand
- BD Phosflow™
- Characteristics
- The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
Clone
- 27-35
- Isotype
- IgG2b
-
Mouse IgG2b isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype control (PE)FACS Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (PerCP)FACS Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (FITC)FACS, IsoC, IF IgG2b FITC
Mouse IgG2b isotype controlFACS, IsoC IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
-
-
: "Rapid isolation of cloned isotype switch variants using fluorescence activated cell sorting." in: Cytometry, Vol. 2, Issue 6, pp. 395-401, (1982) (PubMed).
-
: "Rapid isolation of cloned isotype switch variants using fluorescence activated cell sorting." in: Cytometry, Vol. 2, Issue 6, pp. 395-401, (1982) (PubMed).
-
- Target
- IgG2b
- Abstract
- IgG2b Products
- Synonyms
- IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
- Target Type
- Antibody
- Background
- Synonyms: anti-dansyl
-
You are here:
