Rat IgG2a,IgG2b isotype control (Alexa Fluor 700)
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- Target
- IgG2a,IgG2b
- Reactivity
- Mouse
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Host
- Rat
- Clonality
- Monoclonal
- Conjugate
- Alexa Fluor 700
- Application
- Isotype Control (IsoC), Western Blotting (WB), Immunoprecipitation (IP)
- Purification
- The antibodies are purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solutions are free of unconjugated Alexa Fluor® 700.
- Clone
- 17A2-RB6-8C5-RA3-6B2-Ter-119-M1-70
- Isotype
- IgG2b, IgG2a
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- This two-reagent set contains Alexa Fluor® 700 conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
- Handling Advice
- Protect from prolonged exposure to light. Do not freeze.
- Storage
- 4 °C
- Storage Comment
- The antibody solution should be stored undiluted between 2°C and 8°C.
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- Target
- IgG2a,IgG2b
- Alternative Name
- IgG2b,IgG2a
- Background
- The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Alexa Fluor® 700 Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2, anti-mouse Ly-6G/Ly-6C, clone RB6-8C5, anti-mouse CD11b, clone M1/70, anti-mouse CD45R/B220, clone RA3-6B2, anti-mouse TER-119/Erythroid cells, clone Ter-119. Alexa Fluor® 700 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. Alexa Fluor® 700 anti-mCD3/ Alexa Fluor® 700 anti-mLy-6G(Ly-6C)/ Alexa Fluor® 700 anti-mCD11b/ Alexa Fluor® 700 anti-mCD45R(B220)/ Alexa Fluor® 700 anti-mTer-119 2. Alexa Fluor® 700 Rat IgG2b/Rat IgG2a isotype controls Materials not provided: Cell Staining Buffer (Cat. No. 420201)
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