Mouse IgG1 isotype control (FITC)

Details for Product No. ABIN637572, Supplier: Log in to see
Isotype
  • IgG1
  • Igh-4
  • VH7183
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Ighg1
Host
Mouse
244
83
22
9
3
3
2
2
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
42
42
30
25
9
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Isotype Control (IsoC), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen IgG1 kappa light chain antibody (FITC) was raised in mouse using kapa light chain as the immunogen.
Clone M12637
Isotype IgG1
Characteristics FITC Conjugated Mouse IgG1 Kappa Light Chain Isotype Control antibody
Alternative Names: Monoclonal IgG1 Kappa Light Chain Isotype Control, Anti- IgG1 Kappa Light Chain Isotype Control, IgG1 Kappa Light Chain Isotype Control, Immunoglobulin G1 Kappa Light Chain Isotype Control
Purification Affinity purified
Background The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). A typical antibody is composed of two immunoglobulin heavy chains and two immunoglobulin light chains. There are two types of light chain in humans (as in other mammals), kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2 lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22.
Application Notes FC: 20 µL per 10^6 cells in 100 µL volume
This immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.2, with 0.2 % BSA and 0.09 % NaN3.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Do not freeze.
Storage 4 °C
Storage Comment Store at 4 °C.