anti-Human CSF2 antibody for ELISA

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • GMCSF
  • GM-CSF
  • CSF2
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • CSF
  • colony stimulating factor 2
  • colony stimulating factor 2 (granulocyte-macrophage)
  • CSF2
  • Csf2
Reactivity
Human
209
58
17
7
6
3
3
3
2
1
1
Host
Rabbit
114
113
55
4
2
Clonality
Polyclonal
Conjugate
This CSF2 antibody is un-conjugated
26
10
7
4
3
3
2
2
2
2
2
2
2
Application
ELISA, Neutralization (Neut), Western Blotting (WB)
203
195
59
46
37
31
25
11
10
9
7
7
4
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN465581
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1724740 ELISA WB Mouse IgG1 AA 18-144 Log in to see 3D3 3
1 ABIN1995453 ELISA WB Rabbit IgG AA 18-144 Log in to see Polyclonal 0
1 ABIN2869493 ELISA WB Mouse IgG1 AA 18-144 Log in to see 3D3 0
1 ABIN1100979 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN465667 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN1845500 ELISA WB Mouse IgG1 AA 18-144 Log in to see 0
1 ABIN1838656 ELISA WB Mouse IgG2b, kappa Log in to see 1B3E11 0
1 ABIN467140 ELISA WB Mouse IgG1 AA 18-144 Log in to see 3D3 0
1 ABIN1995449 ELISA ELISA (Detection) Mouse IgG1 AA 18-144 Log in to see 06 0
1 ABIN1824609 ELISA WB HRP Rat IgG2a Log in to see KT35 0
1 ABIN595403 ELISA HRP Mouse IgG1, kappa Log in to see MVCAM A (429) 0
1 ABIN1824264 ELISA Rat IgG2a Log in to see KT37 0
1 ABIN595411 FACS ELISA Biotin Rat IgG2a Log in to see 0
1 ABIN1830087 DB IHC ELISA WB Sheep AA 18-144 Log in to see 0
1 ABIN1822014 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2619551 ELISA WB Biotin Mouse IgG1 Log in to see 0
1 ABIN1827677 ELISA WB Mouse IgG2a, kappa Log in to see 2G9 0
1 ABIN2596812 ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2596811 ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN2596813 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Reactivity Human
(209), (58), (17), (7), (6), (3), (3), (3), (2), (1), (1)
Host Rabbit
(114), (113), (55), (4), (2)
Clonality
Polyclonal
Conjugate This CSF2 antibody is un-conjugated
(26), (10), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Neutralization (Neut), Western Blotting (WB)
(203), (195), (59), (46), (37), (31), (25), (11), (10), (9), (7), (7), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling Images
Specificity Human GM-CSF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human GM-CSF.

Type of Immunogen: Cell extract
Plasmids, Primers & others

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CSF2 / GMCSF / GM-CSF (CSF2 Antibody Abstract)
Background Name/Gene ID: CSF2

Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF
Gene ID 1437
UniProt P04141
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), Neut (0.3 - 0.5 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hGM-CSF (1 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (LS-C104714) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling back to top
Supplier Images
 image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (ABIN465581) anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody
 image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (ABIN465581) anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (Image 2)
 image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (ABIN465581) anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (Image 3)
 image for anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (ABIN465581) anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (Image 4)