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|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Conjugate||This CSF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling Images
E.coli derived recombinant Human GM-CSF.
Type of Immunogen: Cell extract
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling Images back to top
|Alternative Name||CSF2 / GMCSF / GM-CSF (CSF2 Antibody Abstract)|
Name/Gene ID: CSF2
Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF
|Pathways||JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.3 - 0.5 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hGM-CSF (1 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (LS-C104714) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Reconstitution||Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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