anti-Human CSF2 antibody for ELISA (Detection)

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • GMCSF
  • GM-CSF
  • CSF2
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • CSF
  • colony stimulating factor 2
  • colony stimulating factor 2 (granulocyte-macrophage)
  • CSF2
  • Csf2
Epitope
AA 18-144
36
11
11
10
9
7
7
5
4
3
3
2
2
1
1
1
1
Reactivity
Human
212
59
17
7
6
4
4
3
2
1
1
Host
Mouse
117
113
56
4
2
Clonality
Monoclonal
Conjugate
This CSF2 antibody is un-conjugated
27
10
7
4
3
3
2
2
2
2
2
2
2
Application
ELISA, ELISA (Detection)
206
201
59
47
38
32
26
13
10
10
9
8
4
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1995449
$ 218.90
Plus shipping costs $45.00

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Epitope AA 18-144
(36), (11), (11), (10), (9), (7), (7), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(212), (59), (17), (7), (6), (4), (4), (3), (2), (1), (1)
Host Mouse
(117), (113), (56), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is un-conjugated
(27), (10), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, ELISA (Detection)
(206), (201), (59), (47), (38), (32), (26), (13), (10), (10), (9), (8), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling Images
Specificity Human GM-CSF / CSF2
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GM-CSF / CSF2 (rh GM-CSF / CSF2, ABIN2001916 NP_000749.2, Ala 18-Glu 144,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human GM-CSF / CSF2 protein (ABIN2001916)
Clone 06
Isotype IgG1
Plasmids, Primers & others

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CSF2 (CSF2 Antibody Abstract)
Background Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells. GM-CSF is secreted by a variety of cell types including macrophages, T cells, mast cells, endothelial cells and fibroblasts. This cytokine has a central role in governing the proliferation, maturation, differentiation and function of granulocyte and macrophage lineage populations, as well as the migration of endothelial cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target.
NCBI Accession NP_000749.2
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human GM-CSF. The detection limit for Human GM-CSF is approximately 0.16 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months