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|Antigen||Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CSF2 antibody is conjugated to Biotin Alternatives|
ELISA, Neutralization (Neut), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CSF2 AntibodyTarget Details CSF2 Application Details Handling Images
E.coli derived recombinant Murine GM-CSF.
Type of Immunogen: Cell extract
Target Details CSF2Product Details anti-CSF2 Antibody Application Details Handling Images back to top
|Alternative Name||CSF2 / GMCSF / GM-CSF (CSF2 Antibody Abstract)|
Name/Gene ID: CSF2
Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF
|Pathways||JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-CSF2 Antibody Target Details CSF2 Handling Images back to top
Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mGM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine GM-CSF (LS-C104755) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2 Antibody Target Details CSF2 Application Details Images back to top
|Reconstitution||Sterile PBS containing 0.1 % BSA|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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