anti-Mouse (Murine) IL-6 Receptor antibody for Immunohistochemistry
Recommended IL-6 Receptor Antibody
- Antigen
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Interleukin 6 Receptor (IL6R) Antibodies
show synonyms for this antigen- IL6R
- il-6ra
- CD126
- IL-6R-1
- IL-6RA
- IL6Q
- IL6RA
- IL6RQ
- gp80
- IL6R1
- Il6ra
- IL-6Ralpha
- IL-6R
- Il6r
- interleukin 6 receptor
- interleukin 6 receptor, alpha
- IL6R
- il6r
- Il6r
- Il6ra
- Epitope
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AA 20-357
Alternatives - Reactivity
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Mouse (Murine)
Alternatives - Host
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Goat
Alternatives - Clonality
- Conjugate
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This IL-6 Receptor antibody is un-conjugated
Alternatives - Application
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Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
Alternatives - Options
Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Clonality | References | Details |
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10.081744 | ABIN6262530 | ELISA IHC WB | Rabbit | IgG | Polyclonal | 0 | |||
10 | ABIN1175054 | ICC IHC WB | Rabbit | IgG | AA 205-347 | Polyclonal | 0 | ||
10 | ABIN2935539 | IF/ICC IHC IP WB | Rabbit | AA 205-347 | Polyclonal | 0 | |||
7 | ABIN1859439 | ICC IHC WB | Rabbit | IgG | AA 52-204 | Polyclonal | 0 | ||
7 | ABIN2935540 | IF/ICC IHC IP WB | Rabbit | AA 52-204 | Polyclonal | 0 | |||
4 | ABIN2660934 | ELISA (Capture) ELISPOT (Capture) ICFC Neut IHC WB | Biotin | Rat | IgG2b kappa | D7715A7 | 11 | ||
4 | ABIN2609956 | IHC IHC (p) WB | Rabbit | IgG | AA 205-347 | Polyclonal | 0 | ||
1 | ABIN2665905 | FACS IHC | Rat | IgG2b kappa | D7715A7 | 11 | |||
1 | ABIN2665904 | FACS IHC | Rat | IgG2b kappa | D7715A7 | 11 | |||
1 | ABIN2660933 | ELISA (Capture) ELISPOT (Capture) ICFC Neut IHC WB | Biotin | Rat | IgG2b kappa | D7715A7 | 11 | ||
1 | ABIN1175056 | IHC ELISA WB | Biotin | Rabbit | IgG | Polyclonal | 0 | ||
1 | ABIN6052487 | IF/ICC IHC WB | Biotin | Rabbit | IgG | Polyclonal | 0 | ||
-8.685398 | ABIN439445 | IHC (fro) IHC WB | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 1 | ||
-13.185398 | ABIN5606320 | IHC IHC (fro) WB | Alexa Fluor 405 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606322 | IHC IHC (fro) WB | Alexa Fluor 647 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606326 | IHC IHC (fro) WB | DyLight 405 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606323 | IHC IHC (fro) WB | Alexa Fluor 700 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606329 | IHC IHC (fro) WB | DyLight 650 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606325 | IHC IHC (fro) WB | DyLight 350 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 | |
-13.185398 | ABIN5606330 | IHC IHC (fro) WB | DyLight 680 | Rat | IgG2 | Extracellular Domain | RM0114-14F33 | 0 |
General |
|
---|---|
Antigen | Interleukin 6 Receptor (IL6R) Antibodies |
Epitope | AA 20-357 Alternatives |
Reactivity | Mouse (Murine) Alternatives |
Host | Goat Alternatives |
Clonality | |
Conjugate | This IL-6 Receptor antibody is un-conjugated Alternatives |
Application |
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
Alternatives
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10 references available |
Supplier | |
Product Details anti-IL-6 Receptor AntibodyTarget Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images |
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Specificity | Detects mouse IL-6 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3 % cross-reactivity with recombinant mouse (rm) IL-6, recombinant human IL-6 R and rmgp130 is observed. |
Purification | Immunogen affinity purified |
Sterility | 0.2 μm filtered |
Endotoxin Level | <0.10 EU per 1 μg of the antibody by the LAL method. |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha , Leu20-Glu357, Accession # P22272 |
Isotype | IgG |
Plasmids, Primers & others | |
Target Details IL-6 ReceptorProduct Details anti-IL-6 Receptor Antibody Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top |
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Antigen | |
Alternative Name | IL-6 R alpha (IL6R Antibody Abstract) |
Gene ID | 3570 |
UniProt | P22272 |
Pathways | JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints |
Application DetailsProduct Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top |
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Application Notes |
Western Blot 0.1 μg/mL Flow Cytometry 2.5 μg/10^6 cells Immunohistochemistry 5-15 μg/mL Neutralization 0.2-1 μg/mL CyTOF-ready ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL |
Restrictions | For Research Use only |
HandlingProduct Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top |
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Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | Without preservative |
Storage | 4 °C,-20 °C,-80 °C |
Storage Comment | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution. |
Expiry Date | 12 months |
References for anti-IL-6 Receptor antibody (ABIN4899510)Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling Images back to top |
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Product cited in: |
St Leger, Hansen, Karauzum, Horai, Yu, Laurence, Mayer-Barber, Silver, Villasmil, Egwuagu, Datta, Caspi: "STAT-3-independent production of IL-17 by mouse innate-like αβ T cells controls ocular infection." in: The Journal of experimental medicine, Vol. 215, Issue 4, pp. 1079-1090, 2019 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)). Owusu-Boaitey, Bauckman, Zhang, Mysorekar: "Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner." in: Immunity, inflammation and disease, Vol. 4, Issue 4, pp. 413-426, 2018 Method employed by authors: Biochemical Assay (BCA) (Sample species: Mouse (Murine)). Arnold, Boll, Rothaug, Schumacher, Schmidt, Wichert, Schneppenheim, Lokau, Pickhinke, Koudelka, Tholey, Rabe, Scheller, Lucius, Garbers, Rose-John, Becker-Pauly: "Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling." in: Scientific reports, Vol. 7, pp. 44053, 2017 Wang, Miller, Goswami, Zhang, Ronning, Karlen, Zawadzki, Pugh, Burns: "Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 121, 2017 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)). Garbers, Jänner, Chalaris, Moss, Floss, Meyer, Koch-Nolte, Rose-John, Scheller: "Species specificity of ADAM10 and ADAM17 proteins in interleukin-6 (IL-6) trans-signaling and novel role of ADAM10 in inducible IL-6 receptor shedding." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14804-11, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)). Rabe, Chalaris, May, Waetzig, Seegert, Williams, Jones, Rose-John, Scheller: "Transgenic blockade of interleukin 6 transsignaling abrogates inflammation." in: Blood, Vol. 111, Issue 3, pp. 1021-8, 2008 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). Walker, Zhang, Matthews, Weinstock, Nice, Ernst, Rose-John, Burgess: "IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice." in: Blood, Vol. 111, Issue 8, pp. 3978-85, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)). Chalaris, Rabe, Paliga, Lange, Laskay, Fielding, Jones, Rose-John, Scheller: "Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils." in: Blood, Vol. 110, Issue 6, pp. 1748-55, 2007 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). Kurooka, Kaneda: "Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells." in: Cancer research, Vol. 67, Issue 1, pp. 227-36, 2007 Method employed by authors: In vivo Studies (in vivo), In vivo Studies (in vivo), In vivo Studies (in vivo) (Sample species: Mouse (Murine)). Osuchowski, Siddiqui, Copeland, Remick: "Sequential ELISA to profile multiple cytokines from small volumes." in: Journal of immunological methods, Vol. 302, Issue 1-2, pp. 172-81, 2005 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). |
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Supplier Images |