anti-Rat (Rattus) Thrombopoietin antibody for Western Blotting

Recommended Thrombopoietin Antibody (supplied by: Log in to see )

Antigen
Thrombopoietin (THPO) Antibodies
  • MGDF
  • MKCSF
  • ML
  • MPLLG
  • THCYT1
  • TPO
  • Mgdf
  • Ml
  • Mpllg
  • Tpo
  • Tpo1
  • Tpo2
  • Tpo3
  • Tpo4
  • tpo
  • LOC100231762
  • thrombopoietin
  • THPO
  • Thpo
  • thpo
Epitope
AA 22-259
6
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
132
49
18
5
2
2
2
2
1
1
1
1
Host
Rabbit
98
46
28
16
Clonality
Polyclonal
Conjugate
This Thrombopoietin antibody is un-conjugated
13
8
5
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
101
28
13
13
10
10
10
7
6
6
5
2
2
2
1
1
Options
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Catalog No. ABIN5518876
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1084251 ABIN2707174 WB Rabbit Center Log in to see Polyclonal 0
3.1084251 ABIN1871857 ICC IHC WB Rabbit IgG AA 24-266 Log in to see Polyclonal 0
3.1084251 ABIN5692952 ELISA WB Rabbit AA 27-259 Log in to see Polyclonal 0
3.1084251 ABIN2902553 IF/ICC IHC IP WB Rabbit AA 24-266 Log in to see Polyclonal 0
3.1084251 ABIN5545197 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1714401 IF (p) IHC (p) WB Rabbit IgG AA 45-95 Log in to see Polyclonal 1
1 ABIN6265559 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2574344 WB Rabbit IgG AA 24-266 Log in to see Polyclonal 0
1 ABIN2574347 WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2886694 WB Rabbit Log in to see Polyclonal 0
1 ABIN6093147 ELISA WB Rabbit IgG AA 22-194 Log in to see Polyclonal 0
1 ABIN6027658 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN5647289 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5708288 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2992601 WB Rabbit Log in to see Polyclonal 0

General

Antigen Thrombopoietin (THPO) Antibodies
Epitope AA 22-259
(6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(132), (49), (18), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(98), (46), (28), (16)
Clonality
Polyclonal
Conjugate This Thrombopoietin antibody is un-conjugated
(13), (8), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (101), (28), (13), (13), (10), (10), (10), (7), (6), (6), (5), (2), (2), (2), (1), (1)
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Product Details anti-Thrombopoietin Antibody

Target Details Thrombopoietin Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: thrombopoietin
Protein Name: Thrombopoietin
Purification Immunogen affinity purified.
Immunogen E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.
Isotype IgG
Plasmids, Primers & others

Target Details Thrombopoietin

Product Details anti-Thrombopoietin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Thpo (THPO Antibody Abstract)
Background Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

Synonyms: C mpl ligand | MGDF | MKCSF | ML | MPL ligand | MPLLG | THCYT1 | THPO | Thrombopoietin | TPO | P40225
Gene ID 21832
UniProt P40226
Pathways JAK-STAT Signaling, Hormone Activity

Application Details

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody . Electro...
Immunohistochemistry (IHC) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody . Thrombopoietin w...
Immunohistochemistry (IHC) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody . Thrombopoietin w...
Immunohistochemistry (IHC) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody . Thrombopoietin w...
Immunohistochemistry (IHC) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody . Thrombopoietin w...
Immunohistochemistry (IHC) image for anti-Thrombopoietin (THPO) (AA 22-259) antibody (ABIN5518876) IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody . Thrombopoietin w...