IL1R1 ELISA Kit

Catalog No. ABIN1112640

Product Details IL1R1 ELISA Kit

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: For quantitative detection of IL-1RA in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Sensitivity: < 2 pg/mL

Components: 1. One 96-well plate pre-coated with anti-human IL-1RA antibody 2. Lyophilized human IL-1RA standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human IL-1RA antibody (Concentrated): 130 µl.

Material not included: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Application Details

Comment:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-1RA polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-1RA polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IL-1RA amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-1RA can be calculated.

Plate: Pre-coated

Reagent Preparation:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Sample Preparation:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with citrate, heparin or EDTA as the anticoagulant. Centrifuge for 15 min at 1500 x g within 30 min of collection. For eliminating the platelet effect, suggesting that further centrifugation for 10 min at 2-8°C at 10000 x g. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide, Thimerosal (Merthiolate)

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL-1 R alpha (IL1R1 Products)

Synonyms: CD121A ELISA Kit, D2S1473 ELISA Kit, IL-1R-alpha ELISA Kit, IL1R ELISA Kit, IL1RA ELISA Kit, P80 ELISA Kit, CD121a ELISA Kit, CD121b ELISA Kit, IL-iR ELISA Kit, Il1r-1 ELISA Kit, DER4 ELISA Kit, Fit-1 ELISA Kit, Ly84 ELISA Kit, ST2L ELISA Kit, St2 ELISA Kit, St2-rs1 ELISA Kit, T1 ELISA Kit, T1/ST2 ELISA Kit, interleukin 1 receptor type 1 ELISA Kit, interleukin 1 receptor, type I ELISA Kit, interleukin 1 receptor-like 1 ELISA Kit, IL1R1 ELISA Kit, Il1r1 ELISA Kit, Il1rl1 ELISA Kit

Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene which maps to 2q14.2. It is a member of the interleukin 1 cytokine family, and it is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R). Carter et al. (1990) found that IL1RN specifically inhibited IL1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL1-induced corticosterone production in vivo. Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1–receptor antagonist (DIRA), and elevated levels of IL1RN has been found in serum of schizophrenia patients.

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints