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- Target See all HCP products
- HCP (Host Cell Proteins (HCP))
- Protein Type
- Native
- Origin
- E. coli
- Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB)
- Purpose
- E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
- Specificity
- E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
- Characteristics
- Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
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- Application Notes
- Western Blot Dilution: 1:500 - 1:1000
- Comment
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E. coli Host Cell Protein Control Proteins detects over 300 E.coli proteins and is suitable for western blotting and ELISA. Kits are also useful in quality control applications as well as lot release testing. Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 4.0 mg/mL
- Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- HCP (Host Cell Proteins (HCP))
- Alternative Name
- Host Cell Proteins (HCP Products)
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