CXCL1 ELISA Kit
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- Target See all CXCL1 ELISA Kits
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- Binding Specificity
- AA 35-107
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 15.6-1000 pg/mL
- Minimum Detection Limit
- 15.6 pg/mL
- Application
- ELISA
- Purpose
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL1
- Brand
- PicoKine™
- Sample Type
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- Specificity
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Expression system for standard: E.coli
Immunogen sequence: A35-N107 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- <1pg/mL
- Material not included
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
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Expression system for standard: E.coli
Immunogen sequence: A35-N107 - Featured
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- Application Notes
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Comment
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
- Plate
- Pre-coated
- Protocol
- human CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A35-N107) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL1 amount of sample captured in plate.
- Assay Procedure
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL1 standard solution and each sample be measured in duplicate.
- Assay Precision
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- Sample 1: n=16, Mean(pg/ml): 83.8, Standard deviation: 3.6, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 357, Standard deviation: 12.8, CV(%): 3.6
- Sample 3: n=16, Mean(pg/ml): 723, Standard deviation: 24.6, CV(%): 3.4,
- Sample 1: n=24, Mean(pg/ml): 95.9, Standard deviation: 5.85, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 363, Standard deviation: 18.9, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 45.82, CV(%): 6.4
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid multiple freeze-thaw cycles.
- Storage
- -20 °C,4 °C
- Storage Comment
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Expiry Date
- 12 months
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IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." in: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
: "After insufficient radiofrequency ablation, tumor-associated endothelial cells exhibit enhanced angiogenesis and promote invasiveness of residual hepatocellular carcinoma." in: Journal of translational medicine, Vol. 10, pp. 230, (2013) (PubMed).
: "
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IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." in: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
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- Target See all CXCL1 ELISA Kits
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- Alternative Name
- CXCL1 (CXCL1 Products)
- Synonyms
- FSP ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, MGSA ELISA Kit, MGSA-a ELISA Kit, NAP-3 ELISA Kit, SCYB1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, CXCL1 ELISA Kit, Sdf1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, GRO ELISA Kit, Gro ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, CXCL1 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, GRO1 ELISA Kit
- Background
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Protein Function: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. .
Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Synonyms: Growth-regulated alpha protein,C-X-C motif chemokine 1,GRO-alpha(1-73),Melanoma growth stimulatory activity,MGSA,Neutrophil-activating protein 3,NAP-3,GRO-alpha(4-73),GRO-alpha(5-73),GRO-alpha(6-73),CXCL1,GRO, GRO1, GROA, MGSA, SCYB1,
Full Gene Name: Growth-regulated alpha protein
Cellular Localisation: Secreted. - Gene ID
- 2919
- UniProt
- P09341
- Pathways
- Autophagy
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