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CCL6 ELISA Kit

CCL6 Reactivity: Mouse AA 22-116 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1706076
  • Target See all CCL6 ELISA Kits
    CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))
    Binding Specificity
    AA 22-116
    Reactivity
    • 5
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL6/C10
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: G22-A116
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: G22-A116
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

    Plate
    Pre-coated
    Protocol
    mouse CCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL6 has been precoated onto 96-well plates. Standards(E.coli, G22-A116) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL6 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL6 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL6 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 43, Standard deviation: 2.4, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 159, Standard deviation: 7.5, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 630, Standard deviation: 21.4, CV(%): 3.4,
    • Sample 1: n=24, Mean(pg/ml): 44, Standard deviation: 3.6, CV(%): 8.2
    • Sample 2: n=24, Mean(pg/ml): 166, Standard deviation: 12.3, CV(%): 7.4
    • Sample 3: n=24, Mean(pg/ml): 621, Standard deviation: 44.1, CV(%): 7.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL6 ELISA Kits
    CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))
    Alternative Name
    Ccl6 (CCL6 Products)
    Synonyms
    MRP-1 ELISA Kit, Scya6 ELISA Kit, c10 ELISA Kit, Abcc1a ELISA Kit, Avcc1a ELISA Kit, Mrp ELISA Kit, Mrp1 ELISA Kit, Mrp-1 ELISA Kit, Scay6 ELISA Kit, chemokine (C-C motif) ligand 6 ELISA Kit, ATP binding cassette subfamily C member 1 ELISA Kit, Ccl6 ELISA Kit, Abcc1 ELISA Kit
    Background

    Protein Function: CCL6(22-95) and CCL6(23-95) are potent chemoattractants. .

    Background: Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

    Synonyms: C-C motif chemokine 6,Protein C10,Small-inducible cytokine A6,CCL6(22-95),CCL6(23-95),Ccl6,C10, Scya6,

    Full Gene Name: C-C motif chemokine 6

    Cellular Localisation: Secreted.
    Gene ID
    20305
    UniProt
    P27784
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