p53 ELISA Kit

Catalog No. ABIN2042629

Human p53 ELISA Kit Colorimetric assay for quantification of Human p53.

Quick Overview for p53 ELISA Kit (ABIN2042629)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details p53 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-P53, Human
Lyophilized recombinant P53, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human P53 in cell lysates.P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kD) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: P53

Gene ID: 7157

UniProt: K7PPA8

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect