p53 ELISA Kit

Catalog No. ABIN921118

Human p53 ELISA Kit, Colorimetric assay for quantification of Human p53, that has been mentioned in 7+ publications.

Quick Overview for p53 ELISA Kit (ABIN921118)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: AA 1-393

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Lysate

Product Details p53 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human P53 ELISA Kit to quantitate Human TP53 in cell lysates.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: M1-D393

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6%, 4.7%, 7.8%
Inter-Assay Precision (CV%): 6.9%, 8.6%, 8.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for p53 ELISA Kit (ABIN921118)

Hasanzadeh, Baghban, Mokhtarzadeh, Shadjou, Mahboob: "An innovative immunosensor for detection of tumor suppressor protein p53 in unprocessed human plasma and cancer cell lysates." in: International journal of biological macromolecules, Vol. 105, Issue Pt 1, pp. 1337-1348, (2018) (PubMed).

Lu, Lin, Gu, Li, Guo, Wang, Qiu: "Antitumor effects of crocin on human breast cancer cells." in: International journal of clinical and experimental medicine, Vol. 8, Issue 11, pp. 20316-22, (2016) (PubMed).

Srivastava, Narang, Bhatia, Saluja: "Expression of Heat Shock Protein 70 Gene and Its Correlation with Inflammatory Markers in Essential Hypertension." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151060, (2016) (PubMed).

Sun, Yang, Wang, Sun, Dong: "Crocin attenuates cisplatin-induced liver injury in the mice." in: Human & experimental toxicology, Vol. 33, Issue 8, pp. 855-62, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Li, Chu, Gao, Li: "Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 80-9, (2009) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53

Background:

Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton ( kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Gene Full Name: tumor protein p53

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect