TGFB1 ELISA Kit

Catalog No. ABIN2042676

This Rat TGFB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat TGFB1.

Quick Overview for TGFB1 ELISA Kit (ABIN2042676)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details TGFB1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is cross-reactivates with TGF 2, TGF 3, TGF 5 <1 % .

Sensitivity: < 1 pg/mL

Components: 96-well plate pre-coated with anti-TGF beta 1, Rat
Lyophilized recombinant TGF beta 1, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat TGF beta 1 in cell culture supernates, serum, plasma (EDTA) and urine.Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.1 TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.2 TGFbeta1 is also an important mediator of bone remodeling.3 TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.4 TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.5 TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.6 The standard product used in this kit is recombinant TGF 1 with the molecular mass of 25kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGF beta 1

Gene ID: 59086

UniProt: P17246

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints