HSD11B2 ELISA Kit
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- Target See all HSD11B2 ELISA Kits
- HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78-50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.27 ng/mL
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Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2) ELISA Kit
HSD11B2 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Saliva, Tissue Homogenate
Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2) ELISA KitHSD11B2 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2) ELISA KitHSD11B2 Reactivity: Mouse Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- Plate
- Pre-coated
- Protocol
- 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all HSD11B2 ELISA Kits
- HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))
- Alternative Name
- 11-beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) (HSD11B2 Products)
- Synonyms
- MGC81883 ELISA Kit, 11-HSD2 ELISA Kit, AME ELISA Kit, AME1 ELISA Kit, HSD11K ELISA Kit, HSD2 ELISA Kit, SDR9C3 ELISA Kit, 11HSD2 ELISA Kit, HSD11B2 ELISA Kit, hydroxysteroid (11-beta) dehydrogenase 2 L homeolog ELISA Kit, hydroxysteroid 11-beta dehydrogenase 2 ELISA Kit, hsd11b2.L ELISA Kit, HSD11B2 ELISA Kit, Hsd11b2 ELISA Kit
- Pathways
- Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones
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