Cadherin 5 ELISA Kit

Catalog No. ABIN2043041

Product Details Cadherin 5 ELISA Kit

Target: Cadherin 5 (CDH5)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.068 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cadherin 5 (CDH5) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Abstract: CDH5 Products

Synonyms: 7B4 ELISA Kit, AA408225 ELISA Kit, Cd144 ELISA Kit, VE-Cad ELISA Kit, VECD ELISA Kit, VEcad ELISA Kit, Vec ELISA Kit, wu:fc88a07 ELISA Kit, CD144 ELISA Kit, cadherin-5 ELISA Kit, cadherin 5 ELISA Kit, CDH5 ELISA Kit, Cdh5 ELISA Kit, cdh5 ELISA Kit

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2