CHEK1 ELISA Kit

Catalog No. ABIN2043125

Product Details CHEK1 ELISA Kit

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.058 ng/mL

Application Details

Plate: Pre-coated

Protocol: Checkpoint Homolog (CHEK1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Homolog (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Homolog (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Homolog (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Checkpoint Homolog (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Checkpoint Homolog (CHEK1) (CHEK1 Products)

Synonyms: An08g10320 ELISA Kit, AO090003000441 ELISA Kit, CHK1 ELISA Kit, chk1 ELISA Kit, C85740 ELISA Kit, Chk1 ELISA Kit, rad27 ELISA Kit, id:ibd2720 ELISA Kit, zgc:56093 ELISA Kit, CHEK1 ELISA Kit, checkpoint kinase 1 ELISA Kit, serine/threonine-protein kinase chk1 ELISA Kit, serine/threonine-protein kinase Chk1 ELISA Kit, CAMK/CAMKL/CHK1 protein kinase Chk1 ELISA Kit, checkpoint kinase 1 S homeolog ELISA Kit, CHEK1 ELISA Kit, ANI_1_2436074 ELISA Kit, AOR_1_768154 ELISA Kit, PTRG_04183 ELISA Kit, SJAG_01680 ELISA Kit, PAAG_04978 ELISA Kit, MCYG_03290 ELISA Kit, VDBG_03742 ELISA Kit, chek1.S ELISA Kit, Chek1 ELISA Kit, chek1 ELISA Kit

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair