CARTPT ELISA Kit

Catalog No. ABIN2043177

Product Details CARTPT ELISA Kit

Target: CARTPT (CART Prepropeptide (CARTPT))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cocaine And Amphetamine Regulated Transcript (CART) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cocaine And Amphetamine Regulated Transcript (CART), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cocaine And Amphetamine Regulated Transcript (CART) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CARTPT

Target: CARTPT (CART Prepropeptide (CARTPT))

Alternative Name: Cocaine And Amphetamine Regulated Transcript (CART) (CARTPT Products)

Synonyms: CART ELISA Kit, Cart ELISA Kit, CARTPT ELISA Kit, CART prepropeptide ELISA Kit, CARTPT ELISA Kit, Cartpt ELISA Kit

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour