COL12A1 ELISA Kit

Catalog No. ABIN2043189

Product Details COL12A1 ELISA Kit

Target: COL12A1 (Collagen, Type XII, alpha 1 (COL12A1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.134 ng/mL

Application Details

Plate: Pre-coated

Protocol: Collagen Type XII (COL12) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XII (COL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XII (COL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XII (COL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Collagen Type XII (COL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for COL12A1

Target: COL12A1 (Collagen, Type XII, alpha 1 (COL12A1))

Alternative Name: Collagen Type XII (COL12) (COL12A1 Products)

Synonyms: BA209D8.1 ELISA Kit, COL12A1L ELISA Kit, DJ234P15.1 ELISA Kit, AW743884 ELISA Kit, COL12A1 ELISA Kit, col12a1 ELISA Kit, collagen type XII alpha 1 chain ELISA Kit, collagen, type XII, alpha 1 ELISA Kit, collagen, type XII, alpha 1a ELISA Kit, Cuticle collagen 12 ELISA Kit, COL12A1 ELISA Kit, Col12a1 ELISA Kit, col12a1a ELISA Kit, col12a1 ELISA Kit, col-12 ELISA Kit