SLC11A2 ELISA Kit

Catalog No. ABIN2043355

Product Details SLC11A2 ELISA Kit

Target: SLC11A2 (Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 2 (SLC11A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Divalent Metal Transporter 1 (DMT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Divalent Metal Transporter 1 (DMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Divalent Metal Transporter 1 (DMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Divalent Metal Transporter 1 (DMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Divalent Metal Transporter 1 (DMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SLC11A2

Target: SLC11A2 (Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 2 (SLC11A2))

Alternative Name: Divalent Metal Transporter 1 (DMT1) (SLC11A2 Products)

Synonyms: DCT1 ELISA Kit, DMT1 ELISA Kit, NRAMP2 ELISA Kit, Nramp2 ELISA Kit, mk ELISA Kit, van ELISA Kit, Dmt1 ELISA Kit, ATNRAMP2 ELISA Kit, F8G22.4 ELISA Kit, F8G22_4 ELISA Kit, NRAMP metal ion transporter 2 ELISA Kit, dct1 ELISA Kit, dmt1 ELISA Kit, nramp2 ELISA Kit, SLC11A2 ELISA Kit, cb426 ELISA Kit, fa07b10 ELISA Kit, wu:fa07b10 ELISA Kit, zgc:136699 ELISA Kit, solute carrier family 11 member 2 ELISA Kit, solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 ELISA Kit, NRAMP metal ion transporter 2 ELISA Kit, manganese transport protein ELISA Kit, metal transporter Nramp2 ELISA Kit, solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2 ELISA Kit, SLC11A2 ELISA Kit, Slc11a2 ELISA Kit, NRAMP2 ELISA Kit, LOC9327403 ELISA Kit, slc11a2 ELISA Kit

Pathways: Transition Metal Ion Homeostasis, Proton Transport, Positive Regulation of Endopeptidase Activity