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IL12RB1 ELISA Kit

IL12RB1 Reactivity: Human Colorimetric Sandwich ELISA 0.15-10 ng/mL
Catalog No. ABIN2043799
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    IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.15-10 ng/mL
    Minimum Detection Limit
    0.15 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.071 ng/mL
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  • Plate
    Pre-coated
    Protocol
    Interleukin 12 Receptor Beta 1 (IL12Rb1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 1 (IL12Rb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12 Receptor Beta 1 (IL12Rb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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    IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
    Abstract
    IL12RB1 Products
    Synonyms
    Il12rb ELISA Kit, IL12RB1 ELISA Kit, IL-12R ELISA Kit, CD212 ELISA Kit, IL-12R-BETA1 ELISA Kit, IL12RB ELISA Kit, IL-12R[b] ELISA Kit, interleukin 12 receptor subunit beta 1 ELISA Kit, interleukin 12 receptor, beta 1 ELISA Kit, Il12rb1 ELISA Kit, IL12RB1 ELISA Kit
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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