SEMA3B ELISA Kit

Catalog No. ABIN2044509

Product Details SEMA3B ELISA Kit

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.59 ng/mL

Application Details

Plate: Pre-coated

Protocol: Semaphorin 3B (SEMA3B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3B (SEMA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3B (SEMA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3B (SEMA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 3B (SEMA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SEMA3B

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Alternative Name: Semaphorin 3B (SEMA3B) (SEMA3B Products)

Synonyms: LUCA-1 ELISA Kit, SEMA5 ELISA Kit, SEMAA ELISA Kit, SemA ELISA Kit, semaV ELISA Kit, AW208495 ELISA Kit, Semaa ELISA Kit, sema5 ELISA Kit, semaphorin 3B ELISA Kit, semaphorin 3B-like ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B ELISA Kit, SEMA3B ELISA Kit, SEMA3BL ELISA Kit, sema3b ELISA Kit, Sema3b ELISA Kit