SEMA3C ELISA Kit

Catalog No. ABIN2044511

Product Details SEMA3C ELISA Kit

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 13.4 pg/mL

Application Details

Plate: Pre-coated

Protocol: Semaphorin 3C (SEMA3C) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3C (SEMA3C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3C (SEMA3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3C (SEMA3C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 3C (SEMA3C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SEMA3C

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Alternative Name: Semaphorin 3C (SEMA3C) (SEMA3C Products)

Synonyms: SEMAE ELISA Kit, SemE ELISA Kit, 1110036B02Rik ELISA Kit, Semae ELISA Kit, semaphorin 3C ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C ELISA Kit, SEMA3C ELISA Kit, Sema3c ELISA Kit