SPOCK2/Testican 2 ELISA Kit

Catalog No. ABIN2044659

Product Details SPOCK2/Testican 2 ELISA Kit

Target: SPOCK2/Testican 2 (SPOCK2) (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 2 (SPOCK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.132 ng/mL

Application Details

Plate: Pre-coated

Protocol: Testican 2 (TIC2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Testican 2 (TIC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Testican 2 (TIC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Testican 2 (TIC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Testican 2 (TIC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SPOCK2/Testican 2

Target: SPOCK2/Testican 2 (SPOCK2) (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 2 (SPOCK2))

Alternative Name: Testican 2 (TIC2) (SPOCK2 Products)

Synonyms: ON ELISA Kit, testican-2 ELISA Kit, AA407235 ELISA Kit, Gcap26 ELISA Kit, mKIAA0275 ELISA Kit, spock2 ELISA Kit, secreted protein acidic and cysteine rich ELISA Kit, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 2 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2 S homeolog ELISA Kit, SPARC ELISA Kit, SPOCK2 ELISA Kit, Spock2 ELISA Kit, spock2.S ELISA Kit

Pathways: Glycosaminoglycan Metabolic Process