SLC22A12 ELISA Kit

Catalog No. ABIN2044759

Product Details SLC22A12 ELISA Kit

Target: SLC22A12 (Solute Carrier Family 22 (Organic Anion/urate Transporter), Member 12 (SLC22A12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.127 ng/mL

Application Details

Plate: Pre-coated

Protocol: Urate Transporter 1 (URAT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urate Transporter 1 (URAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urate Transporter 1 (URAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urate Transporter 1 (URAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Urate Transporter 1 (URAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SLC22A12

Target: SLC22A12 (Solute Carrier Family 22 (Organic Anion/urate Transporter), Member 12 (SLC22A12))

Alternative Name: Urate Transporter 1 (URAT1) (SLC22A12 Products)

Synonyms: AI987855 ELISA Kit, OAT4L ELISA Kit, Rst ELISA Kit, Slc22al2 ELISA Kit, URAT1 ELISA Kit, RST ELISA Kit, solute carrier family 22 (organic anion/cation transporter), member 12 ELISA Kit, solute carrier family 22 member 12 ELISA Kit, Slc22a12 ELISA Kit, SLC22A12 ELISA Kit