UACA ELISA Kit

Catalog No. ABIN2044767

Product Details UACA ELISA Kit

Target: UACA

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.312-20 ng/mL

Application Details

Plate: Pre-coated

Protocol: Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for UACA

Target: UACA

Alternative Name: Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) (UACA Products)

Synonyms: NUCLING ELISA Kit, 2700059D02Rik ELISA Kit, nucling ELISA Kit, RGD1560011 ELISA Kit, uveal autoantigen with coiled-coil domains and ankyrin repeats ELISA Kit, UACA ELISA Kit, Uaca ELISA Kit

Pathways: Positive Regulation of Endopeptidase Activity