WIF1 ELISA Kit

Catalog No. ABIN2044847

Product Details WIF1 ELISA Kit

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 6.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: WNT Inhibitory Factor 1 (WIF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WIF1

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Abstract: WIF1 Products

Synonyms: shf ELISA Kit, GB10372 ELISA Kit, wif-1 ELISA Kit, WIF1 ELISA Kit, WIF-1 ELISA Kit, AW107799 ELISA Kit, zgc:110507 ELISA Kit, protein shifted ELISA Kit, wnt inhibitory factor 1 ELISA Kit, WNT inhibitory factor 1 ELISA Kit, Wnt inhibitory factor 1 ELISA Kit, WNT inhibitory factor 1 S homeolog ELISA Kit, LOC552805 ELISA Kit, CpipJ_CPIJ003761 ELISA Kit, wif1 ELISA Kit, WIF1 ELISA Kit, Wif1 ELISA Kit, LOC100511326 ELISA Kit, wif1.S ELISA Kit

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation