IL-5 ELISA Kit

Catalog No. ABIN2112000

Product Details IL-5 ELISA Kit

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.12-200 pg/mL

Minimum Detection Limit: 3.12 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.8 pg/mL

Components: MOUSE IL-5 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-5 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
TMB SUBSTRATE
STOP SOLUTION

Application Details

Protocol: Mouse IL-5 is a heamotapoietic cytokine, mainly produced by T-helper 2 cells and mast cells. The cytokine is comprised of two identical 113 amino acid units linked by disulfide bond. The gene for mouse IL-5 is located at chromosome 11 in conjunction with genes for IL4 and other T-helper 2 cytokines such as IL-13, GM-CSF, IL-3. IL-5 regulates B cell proliferation, eosinophil maturation and activation. IL-5 receptor is a heterodimer with a cytokine specific alpha unit, and a signal transduction beta subunit that is identical to the beta subunit of receptors for IL-3 and GM-CSF. IL-5 receptor alpha (IL-5Ra) is expressed by eosinophils, B cells and basophils in member-bound form and soluble form. While the membrane bound form participates in the activation of eosinophils and B cells, the soluble form of IL-5Ra has an antagonist effect to IL-5. Increased IL-5 expression causes chronic IgE response and hypereosinophilic syndromes. Elevated IL-5 level is found in allergic diseases such as allergic rhinitis, asthma and allergic conjunctivitis. This ELISA kit provides a tool for studying IL-5 expression and regulation in animal model. This mouse IL-5 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-5. When standards or samples are added to the appropriate microtiter plate wells, mouse IL-5 in the standards or samples will be immobilized by the precoated antibody during incubation. Then, a biotin-conjugated antibody preparation specific for mouse IL-5 is added to each well and incubated.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin-5 (IL5 Products)

Synonyms: EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour