Anti-Beta1 Adrenergic Receptor Antibodies (IgG) ELISA Kit
Reactivity: Human
Colorimetric
Plasma, Serum
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- Target
- Anti-Beta1 Adrenergic Receptor Antibodies (IgG)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Sample Type
- Serum, Plasma
- Characteristics
- The β1-adrenergic receptor is a G protein-coupled receptor. β receptors have the subtypes β1, β2 and β3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The occurrence of auto- antibodies against the β1-adrenergic receptor is associated with the existence of dilated cardiomyopathy (DCM). Human anti-β1-adrenergic receptor antibody EIA is designed for the determination of antibodies (IgG) against the β1-adrenergic receptor in serum and plasma.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Protocol
- Human anti-β1-adrenergic receptor antibody EIA is an antibody screening test. β1-adrenergic receptors have been pre-coated onto a microtiter plate. During the first incubation the anti-β1 adrenergic receptor antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a HRP labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-β1-adrenergic receptor antibodies.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- Anti-Beta1 Adrenergic Receptor Antibodies (IgG)
- Target Type
- Antibody
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