IL-17 ELISA Kit
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- Target See all IL-17 (IL17) ELISA Kits
- IL-17 (IL17) (Interleukin 17 (IL17))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human Interleukin 17 (IL-17) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 17 (IL-17) concentrations in cell culture supernates, serum, and plasma.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 7 pg/ml
- Characteristics
- Human Interleukin 17 (IL-17) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Plate
- Pre-coated
- Protocol
- Human Interleukin 17 (IL-17) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17 is added to the wells and binds to the combination of capture antibody-IL-17 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-17 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17 standard dilutions and IL-17 sample concentration determined.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all IL-17 (IL17) ELISA Kits
- IL-17 (IL17) (Interleukin 17 (IL17))
- Alternative Name
- IL-17 (IL17 Products)
- Synonyms
- CTLA8 ELISA Kit, IL-17 ELISA Kit, IL-17A ELISA Kit, IL17 ELISA Kit, Ctla-8 ELISA Kit, Ctla8 ELISA Kit, Il17 ELISA Kit, ChIL-17 ELISA Kit, IL-17F ELISA Kit, IL17A ELISA Kit, CTLA-8 ELISA Kit, interleukin 17A ELISA Kit, IL17A ELISA Kit, Il17a ELISA Kit
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