IL-1 beta ELISA Kit

Catalog No. ABIN2648759

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-1 beta.

Quick Overview for IL-1 beta ELISA Kit (ABIN2648759)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details IL-1 beta ELISA Kit

Sensitivity: 2 pg/ml

Characteristics: Interleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes (1), is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes (2,3). After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1a and secreted IL-1b. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1b present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1βis added to the wells and binds to the combination of capture antibody-IL-1βin sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1β present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1β standard dilutions and IL-1β sample concentration determined

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome