TGFB1 ELISA Kit

Catalog No. ABIN2648829

This Human TGFB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TGFB1.

Quick Overview for TGFB1 ELISA Kit (ABIN2648829)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details TGFB1 ELISA Kit

Purpose: Human TNF Beta 1 (TNF-β1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human TNF Beta 1 (TNF-β1 ) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 15 pg/ml

Characteristics: Human TNF Beta 1 (TNF-β1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human TNF Beta 1 (TNF-β1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TGF-β1 is added to the wells and binds to the combination of capture antibody-TGF-β1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TGF-β1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-β1 standard dilutions and TGF-β1 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGF-Beta1

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints