IL12 ELISA Kit

Catalog No. ABIN2690561

Product Details IL12 ELISA Kit

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 - 500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: The human IL-12 (p70) ELISA Kit is for the in vitro quantitative determination of human IL-12 (p70) in serum, plasma, and cell culture supernatant.

Brand: BD OptEIA™

Sample Type: Serum, Plasma, Cell Culture Supernatant

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human IL-12 (p70) ELISA Kit

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for IL12 ELISA Kit (ABIN2690561)

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (IL12 Products)

Synonyms: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, LOC100217488 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12A ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12B ELISA Kit, IL12A ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Background: Human Interleukin-12 (p70) (hIL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein composed of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The BD OptEIA™ kit specifically measures only this biologically active p70.
IL-12 stimulates the growth of activated CD4+ and CD8+ T cells and NK cells. IL-12 also promotes the development of proinflammatory-Th1-like CD4+ cells and cytotoxic CD8+ T cells. Other effects include increasing the lytic activity of NK cells and antibody-dependent cell-mediated cytotoxic cells, synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, and inducing NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome